Sf900ii

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SF900II is a cell culture media designed for insect cell lines. It is a liquid, serum-free, protein-free media that supports the growth and maintenance of a variety of insect cell lines, including Sf9, Sf21, and High Five cells.

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Lab products found in correlation

Pen strep, by Thermo Fisher Scientific (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) Sf9 insect cells, by Thermo Fisher Scientific (1 mentions) M17 broth, by Thermo Fisher Scientific (1 mentions)

6 protocols using sf900ii

Culturing Female Drosophila S2 Cells

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Female Drosophila S2 cells (Zhang et al., 2010 (link)) (Invitrogen) were cultured in Sf-900 II (Life Technologies) + Pen/Strep (Gibco) and split every 3-4 days.

Gambarotto D., Pennetier C., Ryniawec J.M., Buster D.W., Gogendeau D., Goupil A., Nano M., Simon A., Blanc D., Racine V., Kimata Y., Rogers G.C, & Basto R. (2019). Plk4 Regulates Centriole Asymmetry and Spindle Orientation in Neural Stem Cells. Developmental Cell, 50(1), 11-24.e10.

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CDV Expression in Sf9 Cells

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Sf9 insect cells were maintained in SF900II (Life Technologies, San Diego, CA, USA) at 27 °C in cell culture, and CDV H gene was obtained from giant panda/SX/2014. A recombinant Onderstepoort strain expressing green fluorescent protein used for virus neutralization was constructed and generated using a reverse genetic system based on RNA polymerase II for CDV by our laboratory.

Dong J., Chen Y., Shi L., Shen B., Sun X., Ruan K., Xia X., Feng H, & Feng N. (2021). Nanoparticles of conformation-stabilized canine distemper virus hemagglutinin are highly immunogenic and induce robust immunity. Virology Journal, 18, 229.

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Culturing Human Embryonic Kidney and Rat Pheochromocytoma Cells

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Human embryonic kidney (HEK) 293 cells (#CRL-1572, ATCC, Manassas, VA) were grown in Dulbecco's Modification of Eagle's Medium (DMEM; Mediatech, Inc., Manassas, VA) with 4.5 g/l glucose, l-glutamine, sodium pyruvate, and supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Mediatech, Inc.), 100 U/ml penicillin (Mediatech, Inc.), and 0.1 mg/ml streptomycin (Mediatech, Inc.) in a 5% CO₂-humidified incubator at 37°C. PC12 rat pheochromocytoma cells (#CRL-1721, ATCC) were grown in the above medium, except supplemented with 10% horse serum and 5% FBS. SF9 insect cells (#11496015, Life Technologies, Carlsbad, CA) were grown in SF900 II (#10902096, Life Technologies) supplemented with penicillin/streptomycin antibiotics on a shaking platform in a room air incubator at 18°C.

Duellman T., Chen X., Wakamiya R, & Yang J. (2018). Nucleic acid-induced potentiation of matrix metalloproteinase-9 enzymatic activity. Biochemical Journal, 475(9), 1597-1610.

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Influenza Virus Propagation in Eggs

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Sf9 insect cells were maintained in SF900II (Life Technologies, San Diego, CA, USA) at 27 °C in cell culture. Influenza virus A/Aichi/68 H3N2 and A/Anhui/2013 H7N9 provided by Dr. Zhu Hongwei (Ludong University) were propagated in the allantoic cavities of 10-day-old embryonated hen’s eggs at 37 °C for 2 d. Allantoic fluid was harvested and centrifuged at 2000 rpm for 10 min. The viruses were titrated by infection of mice with serial dilutions, and LD₅₀ (50% lethal dose) was calculated using the method of Reed and Muench.

Shi L., Long Y., Zhu Y., Dong J., Chen Y., Feng H, & Sun X. (2023). VLPs containing stalk domain and ectodomain of matrix protein 2 of influenza induce protection in mice. Virology Journal, 20, 38.

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RNAi Knockdown in S2 Cells

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S2 cells were grown in serum free medium (SF900-II, life technologies). Templates for in vitro dsRNA transcription were generated by PCR using oligonucleotides containing T7 RNA polymerase promoter sequences at their 5′ ends (see SI for sequences). dsRNAs were generated by in vitro transcription with T7 RNA polymerase using standard methods. After phenol/chloroform extraction and ethanol precipitation, dsRNAs were added to the medium at a concentration of 10 ug/mL 24h, 48h and 72h before heat shock.

Gardini A., Baillat D., Cesaroni M., Hu D., Marinis J.M., Wagner E.J., Lazar M.A., Shilatifard A, & Shiekhattar R. (2014). Integrator Regulates Transcriptional Initiation and Pause Release Following Activation. Molecular cell, 56(1), 128-139.

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Cultivation of L. lactis and Sf9 cells

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The L. lactis MG1363 strain was grown at 30°C in M17 broth (Oxoid Ltd., Basingstoke, UK) supplemented with 0.5% glucose (GM17). Sf9 insect cells were maintained in SF900II (Life Technologies, San Diego, CA, USA) at 27°C, and suspension cultures were kept at a 27°C incubator while shaking at 120 rpm. A live canine distemper vaccine (CDV-11 strain) was purchased from Qilu Animal Health Products Co., Ltd. (Jinan, China).

Wang J., Liu L., Zong X., Wang C., Zhu G., Yang G., Jiang Y., Yang W., Huang H., Shi C., Zeng Y., Wang N., Cao X., Wang C, & Feng N. (2024). Immunogenicity and protective efficacy of a novel bacterium-like particle-based vaccine displaying canine distemper virus antigens in mice and dogs. Microbiology Spectrum, 12(4), e03477-23.

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