Ab4500 q trap uplc ms ms system

Manufactured by AB Sciex

Sourced in Canada, United Kingdom, United States

The AB4500 Q TRAP UPLC/MS/MS System is an analytical instrument designed for high-performance liquid chromatography and tandem mass spectrometry analysis. It combines an ultra-performance liquid chromatography (UPLC) system with a triple quadrupole linear ion trap mass spectrometer to provide sensitive and selective quantitative and qualitative analysis capabilities.

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Lab products found in correlation

Analyst 1, by AB Sciex (11 mentions) Turbo ion spray interface, by AB Sciex (7 mentions) Scientz 100f, by Ningbo Scientz Biotechnology (2 mentions) Esi turbo ion spray interface, by AB Sciex (1 mentions) Ultra performance liquid chromatography (uplc), by Shimadzu (1 mentions) Nexerax2, by Agilent Technologies (1 mentions) Agilent sb c18 column, by Agilent Technologies (1 mentions) Scaa 104, by ANPEL (1 mentions)

11 protocols using ab4500 q trap uplc ms ms system

Optimization of MS/MS Metabolite Profiling

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The LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), the AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface that was operated in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: an ion source, turbo spray; source temperature, 550°C; ion spray voltage (IS), 5,500 V (positive ion mode)/−4,500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 25 psi, respectively; and the collision-activated dissociation (CAD) was set at high. Instrument tuning and mass calibration were performed with 10 and 100 μM polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were conducted with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Yang S., Liu M., Chu N., Chen G., Wang P., Mo J., Guo H., Xu J, & Zhou H. (2022). Combined transcriptome and metabolome reveal glutathione metabolism plays a critical role in resistance to salinity in rice landraces HD961. Frontiers in Plant Science, 13, 952595.

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Metabolite Profiling by Triple Quadrupole Mass Spectrometry

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LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP; AB4500 Q TRAP UPLC/MS/MS System, Milwaukee, United States) equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode, and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature, 550°C; ion spray voltage (IS), 5,500 V (positive ion mode)/−4,500 V (negative ion mode); ion source, gas I (GSI), gas II (GSII), and curtain gas (CUR) set at 50, 60, and 25.0 psi, respectively; and collision-activated dissociation (CAD), high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as multiple reaction monitoring (MRM) experiments with collision gas (nitrogen) set to medium. Declustering potential (DP) and collision energy (CE) for individual MRM transitions were performed with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Ning K., Hou C., Wei X., Zhou Y., Zhang S., Chen Y., Yu H., Dong L, & Chen S. (2022). Metabolomics Analysis Revealed the Characteristic Metabolites of Hemp Seeds Varieties and Metabolites Responsible for Antioxidant Properties. Frontiers in Plant Science, 13, 904163.

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Quantitative Mass Spectrometry Analysis

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LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion modes and controlled by Analyst 1.6.3 software (AB Sciex, Toronto, ON, Canada). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C; ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Lin X., Huang S., Zhang Q., Zhu S, & Dong X. (2022). Changes in the Primary Metabolites of ‘Fengtang’ Plums during Storage Detected by Widely Targeted Metabolomics. Foods, 11(18), 2830.

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Optimization of LIT and QQQ Scans

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To acquire the linear ion trap (LIT) and triple quadrupole scans, an AB4500 Q TRAP UPLC/MS/MS System was used, equipped with an ESI Turbo Ion-Spray interface, running both in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex, Warrington, UK). The triple quadrupole (QQQ) scans were acquired using collision gas (nitrogen) at a medium setting in multiple reaction monitoring (MRM) experiments. Further optimization of declustering potential (DP) and collision energies (CE) was performed for individual MRM transitions.

Tilahun M., Zhao L., Sun L., Shen Y., Ma L., Callaway T.R., Xu J, & Bu D. (2022). Fresh Phyllanthus emblica (Amla) Fruit Supplementation Enhances Milk Fatty Acid Profiles and the Antioxidant Capacities of Milk and Blood in Dairy Cows. Antioxidants, 11(3), 485.

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Metabolite Profiling Using Triple Quadrupole Mass Spectrometry

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LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were: ion source, turbo spray; source temperature, 550 °C; and ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode). The ion source gas I (GSI), gas II(GSII), and curtain gas (CUR) were set at 50, 60, and 25 psi, respectively. The collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μM polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were measured with further DP and CE optimization. A specific set of MRM transitions was monitored for each period according to the metabolites that were eluted during this period.

Yang S., Chu N., Zhou H., Li J., Feng N., Su J., Deng Z., Shen X, & Zheng D. (2022). Integrated Analysis of Transcriptome and Metabolome Reveals the Regulation of Chitooligosaccharide on Drought Tolerance in Sugarcane (Saccharum spp. Hybrid) under Drought Stress. International Journal of Molecular Sciences, 23(17), 9737.

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Targeted Metabolomics Using Q TRAP

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LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode, and controlled by Analyst 1.6.3 software (AB Sciex, Framingham, MA, USA). The ESI source operation parameters were as follows: ion source, turbo spray; source temperature 550 °C, ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode), ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Lv Y.P., Zhao G., Xie Y.F., Owusu A.G., Wu Y, & Gao J.S. (2023). Transcriptome and Metabolome Profiling Unveil Pigment Formation Variations in Brown Cotton Lines (Gossypium hirsutum L.). International Journal of Molecular Sciences, 24(6), 5249.

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Quantitative Mass Spectrometry Analysis

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LIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). The ESI source operation parameters were as follows: an ion source, turbo spray; 550 °C source temperature; ion spray voltage (IS) 5500 V (positive ion mode)/−4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII) and curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; the collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were performed with 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were carried out with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Li Y., Fan K., Shen J., Wang Y., Jeyaraj A., Hu S., Chen X., Ding Z, & Li X. (2023). Glycine-Induced Phosphorylation Plays a Pivotal Role in Energy Metabolism in Roots and Amino Acid Metabolism in Leaves of Tea Plant. Foods, 12(2), 334.

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Freeze-Drying and Mass Spectrometry Analysis

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Biological samples are freeze-dried by vacuum freeze-dryer (Scientz-100 F).The freeze-dried sample waLIT and triple quadrupole (QQQ) scans were acquired on a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex). QQQ scans were acquired as MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions was done with further DP and CE optimization. A specific set of MRM transitions were monitored for each period according to the metabolites eluted within this period.

Sun Q., Ma L, & Zhu X. (2023). Metabolomics-based exploration the response mechanisms of Saussurea involucrata leaves under different levels of low temperature stress. BMC Genomics, 24, 297.

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Optimized Triple Quadrupole MS Analysis

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Triple quadrupole (QQQ) and LIT were obtained using a triple quadrupole-linear ion trap mass spectrometer (Q TRAP), AB4500 Q TRAP UPLC/MS/MS System, with an ESI Turbo Ion-Spray interface that was placed in positive and negative ion modes and operated using Analyst 1.6.3 software (AB Sciex). The parameters of the ESI source operation included the following: ion source, turbo spray; source temperature, 550°C; ion spray voltage, (IS) 5500 V (positive ion mode)/-4500 V (negative ion mode); ion source gas I (GSI), gas II (GSII), and curtain gas (CUR) were set at 50, 60, and 25.0 psi, respectively; collision-activated dissociation (CAD) was high. Instrument tuning and mass calibration were carried out using 10 and 100 μmol/L polypropylene glycol solutions in QQQ and LIT modes, respectively. QQQ scans were obtained with MRM experiments with collision gas (nitrogen) set to medium. DP and CE for individual MRM transitions were performed using additional optimization of DP and CE. Certain MRM transitions were observed during each time period according to the eluted metabolites within that time frame.

Xu W., Wang Q., Zhang W., Zhang H., Liu X., Song Q., Zhu Y., Cui X., Chen X, & Chen H. (2022). Using transcriptomic and metabolomic data to investigate the molecular mechanisms that determine protein and oil contents during seed development in soybean. Frontiers in Plant Science, 13, 1012394.

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Metabolome Analysis of Maize Pericarp

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The metabolome was analyzed by MetWare (http://www.metware.cn/) based on UPLC (Ultra Performance Liquid Chromatography UPLC)-MS/MS (Tandem mass spectrometry). The pericarp tissues of B73 and fka1-1 at 15 and 20 DAP were used for analysis. After freeze-drying with a vacuum freeze-dryer (Scientz-100F), a 100 mg sample was extracted six times with 1.2 mL of 70% methanol solution, and then the sample was placed in a refrigerator at 4 °C overnight. Following centrifugation at 13,523 g for 10 min, the extracts were filtered (SCAA-104, 0.22 μm pore size; ANPEL, Shanghai) and analyzed with a UPLC system (UPLC, SHIMADZU Nexera X2) using an Agilent SB-C18 column (1.8 µm, 2.1 mm * 100 mm). The flow velocity was 0.35 mL per min at 40 °C; the injection volume was 4 μL. The effluent was alternatively connected to an ESI-triple quadrupole-linear ion trap (QTRAP)-MS (AB4500 Q TRAP UPLC/MS/MS System) equipped with an ESI Turbo Ion-Spray interface, operating in positive and negative ion mode and controlled by Analyst 1.6.3 software (AB Sciex).

Wang H., Huang Y., Li Y., Cui Y., Xiang X., Zhu Y., Wang Q., Wang X., Ma G., Xiao Q., Huang X., Gao X., Wang J., Lu X., Larkins B.A., Wang W, & Wu Y. (2024). An ARF gene mutation creates flint kernel architecture in dent maize. Nature Communications, 15, 2565.

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