PolyQ (Millipore, MAB1574); HTT (Millipore, MAB2166); GAPDH (Thermo, AM4300); GFP (Abcam, ab32146); (HTT (Abcam, ab45169); Tau (Abcam, ab76128); PIK4K2C (Proteintech, 17077–1ap); Tp53 (Cell signaling, 2524); PSMA1 (Hybridoma kindly provided by K.G. Tanaka, University of Tokyo); mTOR P-S2448 (Cell signaling, 5536); mTOR (Cell signaling, 2983); S6K P-T389 (Cell signaling, 9205), S6K (Cell signaling, 2708); TFEB (Cell signaling, 4240); Akt P-S473 (Cell signaling, 4051); Akt P-T308 (Cell signaling, 13038); Akt (Cell signaling, 9272); Atg7 (Cell signaling, 8558); Ulk1 (Cell signaling, 8054), LBPA (Echelon, Z-PLBPA); STAM (Proteintech, 12434–1-AP); Hrs (Proteintech, 10390-1-AP); Vps37B (Proteintech, 15653-1-AP); Tsg101 (Santa Cruz, Sc-7964); EGFR (Cell signaling, 2232); Vps4A (Santa Cruz, sc-393428); CHMP4A (Kindly provided by Phyllis Hanson, University of Michigan); CHMP4B (Kindly provided by Phyllis Hanson, University of Michigan); CHMP4C (Origene, TA890004S); Donkey anti mouse hrp (Jackson immuno-research, 715-035-150); Goat anti rabbit hrp (Jackson immuno-research, 111-035-144); Goat anti mouse 488 (Thermo, A32723); Goat anti rabbit 594 (Thermo, A-11012); Goat anti mouse 647 (Thermo, A32728).
Vps4a
Manufactured by Santa Cruz Biotechnology
Vps4A is a protein that functions as an ATPase and plays a role in membrane trafficking and protein sorting processes within cells. It is involved in the disassembly of multivesicular bodies and the recycling of protein complexes. Vps4A is an important component of the ESCRT (Endosomal Sorting Complexes Required for Transport) machinery.
Automatically generated - may contain errors
Lab products found in correlation
Ps2448 mtor, by Cell Signaling Technology (1 mentions)
S6k p t389, by Cell Signaling Technology (1 mentions)
Chmp4a, by Santa Cruz Biotechnology (1 mentions)
Donkey anti mouse hrp, by Jackson ImmunoResearch (1 mentions)
Tsg101, by Santa Cruz Biotechnology (1 mentions)
Gapdh, by Thermo Fisher Scientific (1 mentions)
Goat anti rabbit hrp, by Jackson ImmunoResearch (1 mentions)
Lv3000, by Olympus (1 mentions)
Alexa conjugated secondary antibody, by Cell Signaling Technology (1 mentions)
2 protocols using vps4a
1
Comprehensive Protein Analyses in Cell Research
2
Immunofluorescence Staining of Exosome Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were grown on glass dishes for 24 h then fixed for 15 min in 4% paraformaldehyde and permeabilized with 0.3% Triton X-100 for 15 min at room temperature. Next, cells were blocked with 10% goat serum for 1 h at room temperature, followed by overnight incubation with primary antibodies at 4°C. After being washed with PBS, the cells were incubated with Alexa-conjugated secondary antibody (Cell Signaling Technology, Inc.) at 37°C for 1 h and then incubated with DAPI at 37°C for 5 min. The cells were visualized and images captured on a confocal microscope (Olympus LV3000; Olympus Corporation). Primary antibodies were: RPL9 (dilution: 1:50; Abcam; cat. no. ab182556), TSG101 (dilution: 3:100; MilliporeSigma; cat. no. SAB2702167), VPS4A (dilution: 1:50; Santa Cruz Biotechnology; cat. no. SC-393428) and Alix (dilution: 1:50; Santa Cruz Biotechnology; cat. no. sc-53540).
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