T sod test kit

TMPZ (purity 99%), soybean phospholipids (purity 99%) and cholesterol (purity 99%) were purchased from Shanghai Jinsong Industry Corp., Ltd (Shanghai, China). Carboxymethylcellulose sodium (CMC-Na) was purchased from Tianjin Guangfu Institute of Fine Chemical Industry. DPPH and 2-thiobarbituric acid (TBA) were obtained from Shanghai yuanye biology science and technology Corp., Ltd (Shanghai, China). FeSO₄∙7H₂O was purchased from Sinopharm Chemical Reagents Co., Ltd. (Shanghai, China). Trichloroacetic acid (TCA) was obtained from Damao Chemical Reagent Factory (Tianjin, China). T-SOD Test kit was acquired from Jiancheng Bioengineering Institute (Nanjing, China). All chemical reagents were of analytical grade, and deionized distilled water was used in all experiments.

A total superoxide dismutase (T-SOD) test kit (hydroxylamine method), a glutathione S-transferase (GST) test kit (colorimetric method), a glutathione reductase (GR) test kit (colorimetric method), and an ascorbate peroxidase (APX) test kit (colorimetric method) (Nanjing Jiancheng Bioengineering Research Institute, Nanjing, China) were used to determine the activity levels of SOD, GST, GR, and APX, respectively.

Paeonol (purity 99%) and Hydrogen peroxide (H₂O₂) were obtained from Shanghai Suyi Chemical Reagent Co., Ltd. (Shanghai, China) and soybean phospholipids (purity 99%), cholesterol (purity 99%), NaOH, and Trichloroacetic acid (TCA) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China), including sulfate heptahydrate. Poloxamer 407 and 188 were purchased from BAST Company. KH₂PO₄ was purchased from Guangxilong Science Co., Ltd. (Guangxi, China) and 1,1-diphenyl-2-picrylhydrazyl (DPPH, 98%), thiobarbituric acid (TBA), and 2,4-Dinitrochlorobenzene (DNCB) were provided by Shanghai Yuanye Biology Science and Technology Co., Ltd. (Shanghai, China). A T-SOD Test kit was acquired from Jiancheng Bioengineering Institute (Nanjing, China). All the reagents were AR grade.