The following adeno-associated virus (AAV) vectors were made to express genes of interest in astrocytes (produced by Obio Technology (Shanghai) Corp., Ltd., Shanghai, China; AAV titers: 0.5–2 × 1013 copies/mL): AAV-GfaABC1D-enhanced green fluorescent protein (EGFP)-3× FLAG-WPRE (AAV-Control), AAV-GfaABC1D-Igf1-6× His-P2A-EGFP-3× FLAG-WPRE (AAV-insulin like growth factor 1 (IGF1)), and AAV-GfaABC1D-Spp1-6× His-P2A-EGFP-3× FLAG-WPRE(AAV-osteopontin (OPN)). Mice received a stereotactic injection with the 2 μL of the AAV mentioned above 14 days prior to ICH induction. Rapamycin (6 mg/kg/day, HY-10219; MedChemExpress, Weehawken, NJ, USA) was administered by intraperitoneal injection, starting immediately after ICH. Mice were orally administered with OSI-906 (40 mg/kg/day, HY-10191; MedChemExpress), starting immediately after ICH.
Hy 10191
Manufactured by MedChemExpress
HY-10191 is a laboratory instrument used for spectrophotometric analysis. It is designed to measure the absorbance or transmittance of light by a sample, which can be used to determine the concentration of a substance in a solution.
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Lab products found in correlation
2 protocols using hy 10191
1
Astrocyte-Targeted Gene Delivery for ICH
2
Optimizing In Vitro Conditions for Kidney Cells
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Conditionally, immortalized HPC referred to in our study were donated by Shandong University. HPC and human glomerular endothelial cells (HGRECs) were normally expanded in 1640 medium supplemented with 1% penicillin–streptomycin, 10% FBS, and 5.6 mM glucose. Mesangial cells (HMCs) and HK-2 cells (a kind of tubular epithelial cell) were cultured in DMEM. To explore the optimal experimental conditions, 2*106 cells were seeded in six-well plates, serum-starved for 24 h, and stimulated with 20 mM or 40 mM glucose for 24 h (cell density: 50%) or 48 h (cell density: 30%). Inhibitor-treated groups were set with different doses of inhibitors: dapagliflozin (Macklin, 461432-26-8; 50 nM, 100 nM, or 250 nM), OSI-906 (IGF1R antagonist, MedChemExpress, HY-10191; 30 nM or 60 nM), and Da+OSI-906 (50 nM + 60 nM).
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