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Bio plex 200 array system

Manufactured by Bio-Rad
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The Bio-Plex 200 array system is a multiplex assay platform that enables the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes spectrally encoded magnetic beads and laser-based detection technology to provide rapid, accurate, and sensitive results.

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Lab products found in correlation

Dynabeads cd8, by STEMCELL (2 mentions) Human high sensitivity cytokine premixed kit b, by R&D Systems (2 mentions) Human cxcl13 quantikine elisa kit, by R&D Systems (2 mentions) Milliplex nhp cytokine premixed 23 plex panel, by Merck Group (2 mentions) Legendplex data analysis software, by BioLegend (1 mentions) Facscanto 2 cytometer, by BD (1 mentions) Bio plex manager 6, by Bio-Rad (1 mentions) Bio plex phosphoprotein detection kit, by Bio-Rad (1 mentions) Bio plex pro assay kit, by Bio-Rad (1 mentions)

7 protocols using bio plex 200 array system

1

Cytokine Profiling of HIV-specific CD8+ T cells

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One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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2

Cytokine Profiling of HIV-specific CD8+ T cells

Cited 1 time
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One million CD8 T cell-depleted PBMCs (Dynabeads CD8, StemCell) were incubated with an HIV Gag peptide pool (1μg/ml/peptide) or left unstimulated and incubated at 37°C in RPMI supplemented with 10% HAB and PenStrep. Cytokine secretion in supernatants was measured 48 hours after stimulation on a Luminex beads array platform, as previously described19 (link), 20 (link). Measurements were performed in duplicates using the Human High Sensitivity Cytokine Premixed Kit B (R&D Systems) for IL-2, IFN-γ, TNF-α, IL-17A, IL-22 and IL-31 or for IL-17F on a Bio-plex 200 array system (Bio-Rad Laboratories) per manufacturer’ s instructions. CXCL13 was measured separately with the Human CXCL13 Quantikine ELISA Kit (R&D Systems), as per manufacturer’s instructions.
Morou A., Brunet-Ratnasingham E., Dubé M., Charlebois R., Mercier E., Darko S., Brassard N., Nganou-Makamdop K., Arumugam S., Gendron-Lepage G., Yang L., Niessl J., Baxter A.E., Billingsley J.M., Rajakumar P.A., Lefebvre F., Johnson R.P., Tremblay C., Routy J.P., Wyatt R.T., Finzi A., Douek D.C, & Kaufmann D.E. (2019). Altered differentiation is central to HIV-specific CD4+ T cell dysfunction in progressive disease. Nature immunology, 20(8), 1059-1070.
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3

Multiplex Plasma Protein Analysis

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To expand the range of proteins detected with the previous method, the concentration of soluble inflammatory markers in plasma was analysed using three multiplex bead-based immunoassays. These cover a wider range of cytokines, chemokines, growth factors and acute-phase protein biomarkers. Globally, 63 distinct plasma proteins (76 analytes due to some duplications across kits) were assessed simultaneously, using the Bio-Plex 200 array system according to the manufacturer's instructions (Bio-Rad). Further details on immune-based multiplex assays and methodology for immunoglobulin quantification, as well as a reduced validation of our proteomic results using ELISA can all be found in the supplementary material.
Enríquez-Rodríguez C.J., Casadevall C., Faner R., Castro-Costa A., Pascual-Guàrdia S., Seijó L., López-Campos J.L., Peces-Barba G., Monsó E., Barreiro E., Cosío B.G., Agustí A, & Gea J. (2024). COPD: systemic proteomic profiles in frequent and infrequent exacerbators. ERJ Open Research, 10(2), 00004-2024.
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4

Multiplex Cytokine Profiling in Plasma

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The concentrations of circulating cytokines, chemokines, and other analytes were assayed using bead-based multiplex technology. Irradiated plasma samples were incubated with magnetic beads from Milliplex NHP cytokine premixed 23-plex panel (EMD Millipore, Billerica, MA) kits according to the recommendations provided. Analytes measured included IL-1β, IL-1 receptor antagonist (IL-1RA), IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12/23 (p40), IL-13, IL-15, IL-17, IL-18, gamma interferon (IFN-γ), granulocyte colony-stimulating factor (G-CSF), granulocyte–macrophage colony-stimulating factor (GM-CSF), monocyte chemoattractant protein 1 (MCP-1), macrophage inflammatory protein 1α (MIP-1α), MIP-1β, tumor necrosis factor alpha (TNF-α), transforming growth factor alpha (TGF-α), soluble CD40 ligand (sCD40L), and vascular endothelial growth factor (VEGF). The concentrations in each plasma sample were measured using a Bioplex-200 array system (Bio-Rad, Hercules, CA).
Cross R.W., Prasad A.N., Woolsey C.B., Agans K.N., Borisevich V., Dobias N.S., Comer J.E., Deer D.J., Geisbert J.B., Rasmussen A.L., Lipkin W.I., Fenton K.A, & Geisbert T.W. (2023). Natural history of nonhuman primates after conjunctival exposure to Ebola virus. Scientific Reports, 13, 4175.
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5

Multiplex Cytokine Profiling Assay

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A high sensitivity bead array assay (Luminex) (Millipore, Billerica, Massachusetts, USA) was used to measure interleukin-10, interleukin-6, TNF-α, IFN-γ, interleukin-2 and interleukin-13 in plasma and cell supernatant according to the manufacturer’s instructions. Plates were read using Bio-Plex 200 array system (Bio-Rad Laboratories, Hercules, California, USA).
Chetty S., Porichis F., Govender P., Zupkosky J., Ghebremichael M., Pillay M., Walker B.D., Ndung’u T., Kaufmann D.E, & Kasprowicz V.O. (2014). Tuberculosis distorts the inhibitory impact of interleukin-10 in HIV infection. AIDS (London, England), 28(18), 2671-2676.
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6

Multiplex Profiling of Plasma Cytokines and Coagulation Markers

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Concentrations of cytokines and other analytes were assayed using bead-based multiplex technology. Irradiated plasmas were incubated with magnetic beads from Milliplex NHP Cytokine Premixed 23-plex Panel (EMD Millipore, Billerica, MA) or ProcartaPlex NHP TGF-beta 1 simplex (eBioscience, Vienna, Austria) kits according to the manufacturer protocols. The concentrations in each plasma sample were measured using a Bioplex-200 array system (BioRad, Hercules, CA).
Plasma levels of coagulation-associated parameters were quantified using a flow-based Legendplex Human Thrombosis 7-plex bead immunoassay (Biolegend, San Diego, CA). Samples were processed in duplicate following the kit instructions to measure concentrations of the following analytes: p-selectin, d-dimers, p-selectin glycoprotein ligand-1 (PSGL-1), tissue plasminogen activator (tPA), soluble CD40-ligand (sCD40L), plasminogen activator inhibitor-1 (PAI-1), and factor IX. Approximately 2,100 events (beads) per sample were collected on a FACS Canto II cytometer (BD Biosciences, San Jose, CA) using BD FACS Diva software and the raw data was analyzed with BioLegend’s cloud-based LEGENDplex™ Data Analysis Software.
Woolsey C., Jankeel A., Matassov D., Geisbert J.B., Agans K.N., Borisevich V., Cross R.W., Deer D.J., Fenton K.A., Latham T.E., Gerardi C.S., Mire C.E., Eldridge J.H., Messaoudi I, & Geisbert T.W. (2020). Immune correlates of postexposure vaccine protection against Marburg virus. Scientific Reports, 10, 3071.
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7

Cytokine and Phospho-Protein Profiling

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Cell-free culture supernatants of human monocytes (50 ml) were assayed for the presence of the indicated cytokines, chemokines, and growth factors using the Bio-Plex Pro assay kit (Bio-Rad). For phospho-IkBa protein, monocyte protein extracts were prepared and analyzed using the Bio-Plex phospho-protein detection kit (Bio-Rad) as per the manufacturer's instructions. All data were collected using the Bio-Plex 200 array system with Luminex xMap Technology and analyzed using Bio-Plex Manager 6.0 (Bio-Rad).
Shalova I.N., Lim J.Y., Chittezhath M., Zinkernagel A.S., Beasley F., Hernández-Jiménez E., Toledano V., Cubillos-Zapata C., Rapisarda A., Chen J., Duan K., Yang H., Poidinger M., Melillo G., Nizet V., Arnalich F., López-Collazo E, & Biswas S.K. (2015). Human monocytes undergo functional re-programming during sepsis mediated by hypoxia-inducible factor-1α. Immunity, 42(3).
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