Diamat

Manufactured by Bio-Rad

Sourced in United States, Italy, Germany

The Diamat is a laboratory equipment designed for the analysis and measurement of hemoglobin and other proteins. It utilizes the principle of ion-exchange chromatography to separate and quantify different forms of hemoglobin, such as HbA1c, within a biological sample. The Diamat provides accurate and reliable data to support clinical decision-making and patient monitoring.

Automatically generated - may contain errors

Lab products found in correlation

Immulite 2000 analyser, by Siemens (1 mentions) Dimension rxl system, by Siemens (1 mentions) Dax 96, by Bayer (1 mentions) Dimension vistaplatform, by Siemens (1 mentions) Nephelometer 2, by Siemens (1 mentions) Array protein system, by Beckman Coulter (1 mentions) Cobas c311 analyzer, by Roche (1 mentions) Dca 2000 analyzer, by Bayer (1 mentions) Serum separator tubes ssts, by BD (1 mentions) Edta containing vacutainer tubes, by BD (1 mentions) Specific elisa kit, by R&D Systems (1 mentions) Beckman 2, by Beckman Coulter (1 mentions) Vacutainer tube, by BD (1 mentions)

Close spelling variants of this product

HPLC Diamat Analyzer Diamat ion exchange HPLC DIAMAT analyzer Diamat automated glycosylated hemoglobin analyzer

19 protocols using diamat

Metabolic Profiling in Fasting Participants

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All participants were examined in the morning after overnight fasting. Serum and plasma were immediately separated and stored at −20 °C in multiple vials for later analysis.
Serum glucose (Dimension RXL system, Dade Behring, Dallas, TX, USA) and serum insulin (IMMULITE 2000 analyser, Siemens Healthcare Diagnostics, Marburg, Germany) levels were measured using immunoenzymatic assays, and glycosylated haemoglobin (HbA1c) levels were determined using high-performance liquid chromatography (DIAMAT, Bio-Rad, Richmond, CA, USA). The normal range for HbA1c was 4.2–6.0%, and the CV at 5.5% was 4.8.
Home blood glucose measurements were performed using the Roche Diagnostics Accu-Chek Aviva Nano^® glucose meter. We transferred logged data to a computer utilising the Roche Diagnostics Accu-Chek Smart Pix^® device (Roche Diabetes Care Italy S.p.A., Monza, Milan, Italy).
Ketonuria was measured using Bayer Ketostix^® urine strips and ketonemia was determined by Abbott MediSense Optium Xceed Meter^®.
Total cholesterol, HDL cholesterol and triglycerides (TG) were measured using routine laboratory methods. Low-density lipoprotein (LDL) cholesterol was calculated using the Friedwald formula: LDL = total cholesterol − HDL cholesterol − TG/2.2 [30 (link)].

Stagi S., Papacciuoli V., Ciofi D., Piccini B., Farello G., Toni S., Ferrari M, & Chiarelli F. (2021). Retrospective Evaluation on the Use of a New Polysaccharide Complex in Managing Paediatric Type 1 Diabetes with Metabolic Syndrome (MetS). Nutrients, 13(10), 3517.

+ Open protocol

+ Expand

BMI, HbA1c, and Type 2 Diabetes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Information on body-mass index (BMI) and glycated haemoglobin (HbA1c) was available, respectively, in 146 (56.4%) and 179 (69.1%) individuals with type 2 diabetes. BMI was calculated by objectively measuring height (with a Harpenden stadiometer) and weight (with a properly calibrated scale), and by dividing weight in kilograms by the square of height in metres. A venous blood sample was drawn in the morning after an overnight fast. Haemoglobin A1c was measured by a high-performance liquid chromatography analyzer (Bio-Rad Diamat, Milan, Italy); the upper limit of normal for the laboratory was 5.8%.
Patients were classified according to BMI categories as normoweight (BMI <25 Kg/m²), overweight (25-29 Kg/m²) or obese (BMI ≥30 Kg/m²). Un-satisfactory glycaemic control was considered at HbA1c ≥7% and patients were classified accordingly in two categories (satisfactory vs. sub-optimal glycaemic control).

De Santi F., Zoppini G., Locatelli F., Finocchio E., Cappa V., Dauriz M, & Verlato G. (2017). Type 2 diabetes is associated with an increased prevalence of respiratory symptoms as compared to the general population. BMC Pulmonary Medicine, 17, 101.

+ Open protocol

+ Expand

Fasting Blood Biomarker Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

After a 12- to 14-hour fasting, blood samples were collected from all participants for the determination of the study parameters. Blood was drown in a 10 mL tube containing EDTA (0.15% final concentration) and in a regular 10 mL tube. After collection, plasma and serum were immediately separated at 2,500 rpm for 30 minutes at 4°C, and aliquots were stored at −80°C until analysis. Fasting plasma glucose and serum creatinine levels were measured with standard automated laboratory methods (Roche Diagnostics, Milan, Italy). Glycated haemoglobin (HbA_1c) was measured using an automated high-performance liquid chromatography (HPLC) analyzer (Diamat: Bio-Rad Laboratories, Milan, Italy); normal range values in our laboratory are 4–6%. Fasting insulin concentration was measured by radioimmunoassay (Diagnostic Corporation, LA, CA, USA).

Russo G.T., Giandalia A., Romeo E.L., Alibrandi A., Horvath K.V., Asztalos B.F, & Cucinotta D. (2014). Markers of Systemic Inflammation and Apo-AI Containing HDL Subpopulations in Women with and without Diabetes. International Journal of Endocrinology, 2014, 607924.

+ Open protocol

+ Expand

Measuring Cardiovascular Risk Factors

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemoglobin A1c was measured by a Diabetes Control Complications Trial (DCCT)-aligned method, with an automated high-performance liquid chromatography analyzer (Bio-Rad Diamat, Milan, Italy). Serum total cholesterol, HDL-cholesterol and triglycerides were determined by standard laboratory procedures (DAX 96; Bayer Diagnostics, Milan, Italy). LDL-cholesterol was calculated using Friedewald’s equation^{47 (link)}.

Bacchi E., Cavedon V., Zancanaro C., Moghetti P, & Milanese C. (2017). Comparison between dual-energy X-ray absorptiometry and skinfold thickness in assessing body fat in overweigh/obese adult patients with type-2 diabetes. Scientific Reports, 7, 17424.

+ Open protocol

+ Expand

HbA1c Measurement in Type 1 Diabetes

Check if the same lab product or an alternative is used in the 5 most similar protocols

HbA_1c, a more appropriate indicator of overall glucose management in the type 1 diabetes population, was used instead of the LS7 metric of fasting blood glucose. Stable HbA₁ was measured by ion-exchange chromatography (Isolab, Akron, OH, USA) for the first 18 months of EDC, and subsequently by automated high-performance liquid chromatography (Diamat; Bio-Rad, Hercules, CA, USA). The two assays were highly correlated (r=0.95). HbA₁ values were converted to DCCT-aligned HbA_1c values using a regression equation derived from duplicate assays [DCCT HbA_1c = 0.14 + 0.83 (EDC HbA₁)]. Consistent with the current ADA guidelines for glycaemic targets, the ideal range for HbA_1c was <53 mmol/mol (<7%) [27 (link)], intermediate was 53–75 mmol/mol (7–8.9%) and poor was ≥75 mmol/mol (≥9%).

Devaraj S.M., Kriska A.M., Orchard T.J., Miller R.G, & Costacou T. (2020). Cardiovascular health in early adulthood predicts the development of coronary heart disease in individuals with type 1 diabetes: 25 year follow-up from the Pittsburgh Epidemiology of Diabetes Complications study. Diabetologia, 64(3), 571-580.

+ Open protocol

+ Expand

Molecular Basis of α-Thalassemia in Southern Italy

Check if the same lab product or an alternative is used in the 5 most similar protocols

In a project on the molecular basis of α-thalassemia in Southern Italy, the thalassemia centers collaborating in this study selected families/single patient showing an MCV lower than 80 fL associated with normal Hb A2 and iron status. We report the results of the study on 14 carriers belonging to 6 unrelated families living in Sicilia and Puglia. Ethical approval of the protocol was obtained from the Comitato Etico Università Federico II (307/2016; 225/2019).
The hematological data (Table 1), serum iron, ferritin, and total and indirect bilirubin were obtained by standard methods in the collaborating hospitals. Hb level measurement was performed by the cation exchange high-performance liquid chromatography (HPLC) (Bio-Rad, Diamat, or Variant System Hercules, CA, USA). The research activities were carried out inspired by the principles of quality, and by developing guidelines for the protocols to increase the reliability and reproducibility of the results [23 (link),24 (link)].

Cardiero G., Musollino G., Prezioso R., Nigro V, & Lacerra G. (2023). Alpha-Thalassemia in Southern Italy: Characterization of Five New Deletions Removing the Alpha-Globin Gene Cluster. International Journal of Molecular Sciences, 24(3), 2577.

+ Open protocol

+ Expand

Comprehensive Hematological Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hematological parameters, ferritin, transferrin, serum iron, zinc protoporphyrin (Zpp), and bilirubin (total and indirect) were determined by standard methods in the collaborating hospitals. The analysis of hemoglobin (Hb) was performed by cation exchange high performance liquid chromatography (HPLC) (Bio-Rad, Diamat or Variant System Hercules, CA, USA). Heinz body formation and thermal and isopropanol Hb stability tests were performed by standard methods.

Cardiero G., Musollino G., Friscia M.G., Testa R., Virruso L., Di Girgenti C., Caldora M., Colella Bisogno R., Gaudiano C., Manco G, & Lacerra G. (2020). Effect of Mutations on mRNA and Globin Stability: The Cases of Hb Bernalda/Groene Hart and Hb Southern Italy. Genes, 11(8), 870.

+ Open protocol

+ Expand

Hematological Assessment Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hematological parameters, in blood zinc protoporphyrin (ZPP), and in serum ferritin, transferrin, and bilirubin (total and indirect) were determined by standard procedures in the collaborating hospitals.
The hemoglobin (Hb) analysis was performed via cation exchange high-performance liquid chromatography (HPLC) (Bio-Rad, Diamat or Variant System Hercules, CA, USA). Heinz body formation and thermal and isopropanol Hb stability tests were performed via standard methods.

Cardiero G., Musollino G., Prezioso R, & Lacerra G. (2021). mRNA Analysis of Frameshift Mutations with Stop Codon in the Last Exon: The Case of Hemoglobins Campania [α1 cod95 (−C)] and Sciacca [α1 cod109 (−C)]. Biomedicines, 9(10), 1390.

+ Open protocol

+ Expand

Comprehensive Metabolic Panel Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total triglycerides, total cholesterol, serum glucose, white blood cell counts,
and high sensitivity C-reactive protein were determined on a Dimension VISTA
platform (Siemens Healthcare Diagnostics, Eschborn, Germany). Hemoglobin A1c
concentrations were measured using high-performance liquid chromatography
(Bio-Rad Diamat, Munich, Germany).

Pietzner M., Budde K., Rühlemann M., Völzke H., Homuth G., Weiss F.U., Lerch M.M, & Frost F. (2021). Exocrine Pancreatic Function Modulates Plasma Metabolites Through Changes in Gut Microbiota Composition. The Journal of Clinical Endocrinology and Metabolism, 106(5), e2290-e2298.

+ Open protocol

+ Expand

Biomarkers of Cardiovascular Risk

Check if the same lab product or an alternative is used in the 5 most similar protocols

Non-fasting blood samples were taken and serum samples were analyzed within 1 h or stored at – 80^°C. Total, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol were measured photometrically (Hitachi 704, Roche, Mannheim, Germany). Serum triglycerides were determined enzymatically using reagents from Roche Diagnostics (Hitachi 717; Roche, Mannheim, Germany). High-sensitive C-reactive protein (hs-CRP) was determined immunologically on a Behring Nephelometer II with commercially available reagents (Dade Behring, Eschborn, Germany). HbA1c measured in whole blood was determined by high-performance liquid chromatography (Bio-Rad Diamat, Munich, Germany).

Pitchika A., Markus M.R., Schipf S., Teumer A., Van der Auwera S., Nauck M., Dörr M., Felix S., Grabe H.J., Völzke H, & Ittermann T. (2022). Effects of Apolipoprotein E polymorphism on carotid intima-media thickness, incident myocardial infarction and incident stroke. Scientific Reports, 12, 5142.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!