GC specimens were fixed with 10% formaldehyde and then embedded in paraffin. After preparing 4-μm-thick continuous paraffin sections, immunohistochemistry was performed using anti-VDAC3 (1:200, Proteintech, USA), anti-WDR5 (1:200, Cell Signaling Technology, USA), and anti-FBXW7 (1:300, Proteintech, USA) antibodies. The results were obtained with an automatic digital slide scanning and analysis system (Aperio VERSA 8, Germany), and the immune response score (IRS) was calculated26 (link).
Anti vdac3
Manufactured by Proteintech
Sourced in United States
Anti-VDAC3 is a primary antibody that recognizes the Voltage Dependent Anion Channel 3 (VDAC3) protein. VDAC3 is a member of the VDAC family, which are channel-forming integral membrane proteins involved in the regulation of mitochondrial function.
Automatically generated - may contain errors
Lab products found in correlation
Anti fbxw7, by Proteintech (1 mentions)
Anti wdr5, by Cell Signaling Technology (1 mentions)
Rabbit anti hpv16 e7, by Biorbyt (1 mentions)
Rabbit anti vdac1, by Proteintech (1 mentions)
Anti vdac2, by Proteintech (1 mentions)
Image pro plus, by Media Cybernetics (1 mentions)
Cellsens dimension, by Olympus (1 mentions)
2 protocols using anti vdac3
1
Immunohistochemical Analysis of GC Tissue
2
Immunohistochemical analysis of VDAC protein expression
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IHC reactions were performed on TMA slides as described elsewhere. The TMA slides were incubated overnight in a humidified chamber at 4°C with primary antibodies: rabbit anti- VDAC1, anti-VDAC2, and anti-VDAC3 (1:300, Proteintech, USA) and rabbit anti-HPV16 E7 (1:200, Biorbyt). Antibody detection was performed with 3,3′-diaminobenzidine (DAB). CellSens Dimension (version 1.8.1, Olympus) were used to photograph the images and the staining intensity was measured with ImagePro Plus (version 6.0, Media Cybernetics).
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