G csf elisa kit

Manufactured by R&D Systems

The G-CSF ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed to measure the concentration of granulocyte colony-stimulating factor (G-CSF) in human serum, plasma, and cell culture supernatants. The kit uses a specific antibody coated on a microplate to capture G-CSF from the samples, and a detection antibody conjugated with an enzyme to quantify the bound G-CSF through a colorimetric reaction.

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Lab products found in correlation

Mouse il 1β elisa kit, by Thermo Fisher Scientific (1 mentions) Mouse il 6 elisa kit, by Thermo Fisher Scientific (1 mentions) Fluostar omega, by BMG Labtech (1 mentions) Anti mouse ly6g, by BioXCell (1 mentions) Anti mouse il 6, by BioXCell (1 mentions) Corticosterone elisa, by R&D Systems (1 mentions)

Close spelling variants of this product

Human G-CSF Quantikine ELISA Kit Mouse G-CSF Quantikine ELISA kit Human G‐CSF DuoSet ELISA kit G-CSF ELISAs

3 protocols using g csf elisa kit

Cytokine Profiling in Organ Lysates

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Organs were homogenized with a tissue homogenizer in lysis buffer: PBS containing 1x proteinase inhibitors (Sigma-Aldrich) and 0.1% Nonidet P-40 (NP40; Sigma). Cytokine and chemokine analysis from organ lysates and plasma were performed with the mouse 36-plex ProcartaPlex cytokine/chemokine array kit (ThermoFisher Scientific), G-CSF mouse or human ProcartaPlex Simplex Kit (ThermoFisher Scientific), G-CSF ELISA kit (R&D Systems), IL-6 mouse ELISA kit (ThermoFisher Scientific) and IL-1β mouse ELISA kit (ThermoFisher Scientific), following manufacturer’s instructions. All ProcartaPlex samples were analysed using the Luminex Bio-Plex 200 system. ELISA plate results were measured with light absorbance at 450 nm using a plate reader (Fluostar Omega, BMG labtech).

Ioannou M., Hoving D., Aramburu I.V., Temkin M.I., De Vasconcelos N.M., Tsourouktsoglou T.D., Wang Q., Boeing S., Goldstone R., Vernardis S., Demichev V., Ralser M., David S., Stahl K., Bode C, & Papayannopoulos V. (2022). Microbe capture by splenic macrophages triggers sepsis via T cell-death-dependent neutrophil lifespan shortening. Nature Communications, 13, 4658.

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Cytokine Profiling of Tumor Samples

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Tumor supernatants, TRAMP tumor cell CM, and serum were collected and analyzed using a G-CSF ELISA kit (R&D Systems). Millipore mouse cytokine/chemokine panel LI (MPXMCYTO-70K) (Millipore, Billerica, MA) was used for detection of cytokines and chemokines. Expression levels of cytokines/chemokines were normalized to total protein levels.

Yu H., Liu Y., McFarland B.C., Deshane J.S., Hurst D.R., Ponnazhagan S., Benveniste E.N, & Qin H. (2015). SOCS3 Deficiency in Myeloid Cells Promotes Tumor Development: Involvement of STAT3 Activation and Myeloid-Derived Suppressor Cells. Cancer immunology research, 3(7), 727-740.

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Cytokine and Stress Hormone Analysis

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Anti-mouse IL-6 (Bio-X-Cell, BE0046) and anti-mouse Ly6G (Bio-X-Cell, BE0075–1); G-CSF ELISA kit (R&D Systems, MCS00); Corticosterone ELISA (R&D Systems, KGE009); IL-6 ELISA (Biolegend, 431304); Histamine ELISA (Beckman Coulter, IM2015).

Cronin S.J., Tejada M.A., Song R., Laval K., Cikes D., Ji M., Brai A., Stadlmann J., Novatchikova M., Perlot T., Ali O.H., Botta L., Decker T., Lazovic J., Hagelkruys A., Enquist L., Rao S., Koyuncu O.O, & Penninger J.M. (2023). Pseudorabies virus hijacks DDX3X, initiating an addictive “mad itch” and immune suppression, to facilitate viral spread. bioRxiv.

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