Paxgene dna tubes

Manufactured by Preanalytix

Sourced in Switzerland

The PAXgene DNA tubes are designed for the collection, stabilization, and transport of whole blood samples for DNA extraction and analysis. The tubes contain a proprietary reagent that preserves the DNA in the collected sample, enabling reliable downstream processing and testing.

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Lab products found in correlation

Truseq sample preparation kit, by Illumina (1 mentions) Hiseq 2500, by Illumina (1 mentions) Qiaamp system, by Qiagen (1 mentions) Leukolock filter, by Thermo Fisher Scientific (1 mentions) Flexigene dna kit, by Qiagen (1 mentions) Agilent 2200 tapestation, by Agilent Technologies (1 mentions) Paxgene blood dna kit, by Qiagen (1 mentions)

Close spelling variants of this product

PAXgene tubes (53 citations) PaxGene Blood DNA tubes (9 citations)

5 protocols using paxgene dna tubes

Whole Exome Sequencing (WES) Workflow

Cited 4 times

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Detailed procedures for WES have been described previously [16 (link), 17 (link), 19 (link), 20 (link)]. In brief, whole blood samples were preserved using Paxgene DNA tubes (PreAnalytiX, Hombrechtikon, CH), and genomic DNA was extracted using the QIAamp system (Qiagen, Valencia, CA). Enriched exome libraries were captured using a variety of Agilent SureSelect systems according to the manufacturer’s instructions (Agilent, Santa Clara, CA). Final libraries were generated using Illumina TruSeq sample preparation kits and underwent 100 bp paired-end sequencing on a HiSeq 2500 (Illumina, San Diego, CA). Samples were sequenced to a median coverage of 98X in combined studies.

Salfati E.L., Spencer E.G., Topol S.E., Muse E.D., Rueda M., Lucas J.R., Wagner G.N., Campman S., Topol E.J, & Torkamani A. (2019). Re-analysis of whole-exome sequencing data uncovers novel diagnostic variants and improves molecular diagnostic yields for sudden death and idiopathic diseases. Genome Medicine, 11, 83.

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Blood Collection and Preservation

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Approximately 8.5 mL of veinous blood was collected in PAXgene DNA tubes (PreAnalytix) and shipped overnight from the clinical site to OncoSec Medical at ambient temperature. Samples were then allowed to cool to 4°C before storage at −80°C.

Algazi A.P., Twitty C.G., Tsai K.K., Le M., Pierce R., Browning E., Hermiz R., Canton D.A., Bannavong D., Oglesby A., Francisco M., Fong L., Pittet M.J., Arlauckas S.P., Garris C., Levine L.P., Bifulco C., Ballesteros-Merino C., Bhatia S., Gargosky S., Andtbacka R.H., Fox B.A., Rosenblum M.D, & Daud A.I. (2020). Phase II Trial of IL-12 Plasmid Transfection and PD-1 Blockade in Immunologically Quiescent Melanoma. Clinical cancer research : an official journal of the American Association for Cancer Research, 26(12), 2827-2837.

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Blood Sample Pretreatment and RNA Extraction

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Peripheral blood samples were collected at baseline, and tubes were frozen using a sequential freezing process. DNA was extracted from PAXgene DNA tubes (PreAnalytix) using a modified version of the Qiagen FlexiGene DNA kit, and was stored at -20°C. Whole blood for RNA was collected in EDTA tubes and filtered using LeukoLOCK filters (Life Technologies). Total RNA was extracted using a modified version of the LeukoLOCK Total RNA Isolation System protocol, and included DNase treatment to remove genomic DNA. RNA quality was assessed using the Agilent 2200 Tapestation, and only samples with RNA Integrity Number (RIN) ≥ 6.0 were used.

Fuh S.C., Fiori L.M., Turecki G., Nagy C, & Li Y. (2023). Multi-omic modeling of antidepressant response implicates dynamic immune and inflammatory changes in individuals who respond to treatment. PLOS ONE, 18(5), e0285123.

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Epidural Analgesia Blood Sampling

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A single whole blood sample will be obtained, preferentially via an indwelling intravenous cannula inserted as part of routine care for women receiving epidural analgesia after obtaining informed consent. Recruitment is permitted up to 4 hours after placement of the epidural catheter. In the event that a blood sample cannot be obtained in this manner, participants will be asked to consent to obtaining a sample via venipuncture. The blood samples will be stored in Paxgene DNA tubes (PreAnalytix, Switzerland), which facilitates substantially higher DNA yields compared to saliva samples. 22 (link) The samples will be anonymised through the allocation of a unique identification number so that no patient information will be identifiable. Samples will be stored at -80ºC until the final participant has been recruited, at which point DNA will be extracted for Taqman SNP genotyping 23 by laboratory personnel masked to all clinical details.

Ali A., Weber V., Thompson S.J., Abbott T.E.F., Harmston R., David A.L, & Ackland G.L. (2022). IL-1ra polymorphisms and risk of epidural-related maternal fever (EPIFEVER-2): study protocol for a multicentre, observational mechanistic cohort study. International journal of obstetric anesthesia, 50.

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Genomic DNA Extraction from Blood and Saliva

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Genomic DNA was extracted from peripheral blood or saliva. PAXgene DNA tubes (PreAnalytiX GmbH) were used for peripheral blood sampling, and genomic DNA was extracted using a PAXgene Blood DNA kit (Qiagen) according to the manufacturer's instructions. Salivary DNA was collected using an Oragene DNA Sample Collection Kit (Oragene-DNA, Genotek).

Kageyama H., Miyajima M., Ogino I., Nakajima M., Shimoji K., Fukai R., Miyake N., Nishiyama K., Matsumoto N, & Arai H. (2016). Panventriculomegaly with a wide foramen of Magendie and large cisterna magna. Journal of neurosurgery, 124(6).

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