Rabbit anti mouse cd11b

Manufactured by Abcam

Sourced in United Kingdom

Rabbit anti-mouse CD11b is a primary antibody that specifically binds to the CD11b antigen expressed on the surface of mouse myeloid cells, including monocytes, macrophages, and neutrophils. CD11b is a component of the integrin receptor Mac-1 (CD11b/CD18), which plays a role in cell adhesion and migration. This antibody can be used for the identification and characterization of mouse myeloid cells in various applications, such as flow cytometry and immunohistochemistry.

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Lab products found in correlation

Goat anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Dab staining kit, by Maixin Group (1 mentions) Ecl western blotting detection reagent, by GE Healthcare (1 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (1 mentions) Rabbit anti human gapdh, by Cell Signaling Technology (1 mentions) Chemidoc mp imaging device, by Bio-Rad (1 mentions) Alexa fluor 594 goat anti rat igg, by Thermo Fisher Scientific (1 mentions) Rat anti mouse cd68, by Abcam (1 mentions) Axiocam mrc, by Zeiss (1 mentions) Axioskop 40, by Zeiss (1 mentions) Rat anti mouse gr 1, by BD (1 mentions)

Close spelling variants of this product

CD11b (166 citations) Anti-CD11b (60 citations) Rabbit anti-CD11b (19 citations) Mouse anti-CD11b (12 citations) Rabbit anti- (5 citations) Mouse anti (2 citations) Rabbit anti-mouse CD11b antibody (2 citations)

3 protocols using rabbit anti mouse cd11b

Immunohistochemical Analysis of Tumor-Infiltrating Cells

Cited 1 time

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Tumors harvested from the 4T1^WT, 4T1^NC, and 4T1^IL-6low mice models were fixed in 10% formaldehyde and embedded in paraffin. Tissue sections (4 µm) were deparaffinized in xylene and rehydrated through graded concentrations of ethanol. Antigens were retrieved and endogenous peroxidase activities were quenched as described previously (25 (link)). The tumor tissues were incubated overnight at 4°C with rabbit anti-mouse CD11b (1:500), Gr-1 (1:200), and CD3 (1:100) primary antibodies (Abcam, Cambridge, UK). A biotinylated secondary antibody, goat anti-mouse IgG (Santa Cruz Biotechnology, USA), was labeled with streptavidin–horseradish peroxidase (HRP) using a DAB staining kit (Maixin Biotechnology, China). Five high-power fields (400× magnification) for each tissue section were selected randomly for histological evaluation.

Zhang W., Jiang M., Chen J., Zhang R., Ye Y., Liu P., Yu W, & Yu J. (2018). SOCS3 Suppression Promoted the Recruitment of CD11b+Gr-1−F4/80−MHCII− Early-Stage Myeloid-Derived Suppressor Cells and Accelerated Interleukin-6-Related Tumor Invasion via Affecting Myeloid Differentiation in Breast Cancer. Frontiers in Immunology, 9, 1699.

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Western Blot Analysis of NLRP6 Protein

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The tissue was rinsed in saline and processed in RIPA or urea buffers. Protein samples were separated on SDS–PAGE gels under reducing conditions and transferred to nitrocellulose membranes, blocked for 1 h in 5% BSA or milk proteins diluted in PBS or TBS containing 0.5% or 0.1% Tween 20, respectively. Following blocking, membranes were incubated at 4°C overnight with the following antibodies: rabbit anti-mouse Nlrp6 (E-20; Santa Cruz), mouse anti-human NLRP6 (Clint-1; Adipogen), rabbit anti-human GAPDH (Cell Signaling), mouse anti-human tubulin (Sigma-Aldrich), mouse anti-human NLRP6 (R&D Systems), rabbit anti-GFP (Thermo Fisher Scientific), rabbit anti-mouse CD11b (Abcam), and mouse anti-mouse SMA (Clone 1A4; Sigma-Aldrich). Membranes were incubated for 1 h at room temperature with the secondary antibody at 1:5,000 in blocking buffer and visualized using ECL Western blotting detection reagents (Amersham, GE Healthcare). Images were captured using a ChemiDoc MP imaging device (Bio-Rad Laboratories).

Bracey N.A., Platnich J.M., Lau A., Chung H., Hyndman M.E., MacDonald J.A., Chun J., Beck P.L., Girardin S.E., Gordon P.M, & Muruve D.A. (2020). Tissue-selective alternate promoters guide NLRP6 expression. Life Science Alliance, 4(3), e202000897.

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Immunofluorescent Staining of Wound Cells

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For immunofluorescent staining of macrophages, neutrophils, and myofibroblasts, 8μm frozen tissue sections were air-dried, fixed in cold acetone for 10 min, and blocked with 10% goat serum for 30 min. Sections were then incubated with rat anti-mouse CD68 (Abcam, Cambridge, MA), rat anti-mouse Gr-1 (BD Biosciences, San Jose, CA), rabbit anti-mouse α-smooth muscle actin (α-SMA) (for myofibroblast staining, Abcam, Cambridge MA) or rabbit anti-mouse CD11b (Abcam, Cambridge, MA) for 45 min followed by incubation with Alexa fluor 594 goat anti-rat IgG, Alexa fluor goat anti-rat or rabbit IgG 488 (Invitrogen, Carlsbad, CA), respectively. The staining procedures were all performed at room temperature. Stained sections were evaluated using a fluorescence microscope, Axioskop 40 (Zeiss, Oberkochen, Germany) and recorded with a digital camera, AxioCam MRc (Zeiss, Oberkochen, Germany). Gr-1 positive cells in the wounds and wound margins were counted and the average number per 20× field was calculated. The density (% positive staining in wound margin and wound bed) of CD68 and α-SMA was quantified using Image J.^{21 (link)} N = 6 in each group.

Chen L., Nagaraja S., Zhou J., Zhao Y., Fine D., Mitrophanov A.Y., Reifman J, & DiPietro L.A. (2017). Wound healing in Mac-1 deficient mice. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 25(3), 366-376.

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