Charcoal meal

Manufactured by Merck Group

Sourced in United States, Germany

Charcoal meal is a type of laboratory equipment used for the adsorption of various substances. It is a finely ground form of activated carbon, which has a high surface area and porous structure, allowing it to effectively adsorb a wide range of molecules and compounds.

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Lab products found in correlation

9 protocols using charcoal meal

Gastrointestinal Transit Ratio Assay

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The gastrointestinal transit ratio was measured using the method described elsewhere [49 (link)]. Briefly, 24-week-old WT and Lep KO mice were fasted for 18 h before the experiment, but were given access to water ad libitum. Each mouse in the subset group was fed 1 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co., St. Louis, MO, USA). After 30 min of treatment, the mice were euthanized with CO₂, and the intestinal tract was collected from the abdominal cavity. The intestinal charcoal transit ratio was estimated by simple calculation method as follows

The total intestinal length was also measured from the stomach to the anus in duplicate.

Kim J.E., Choi Y.J., Lee S.J., Gong J.E., Lim Y., Hong J.T, & Hwang D.Y. (2020). Molecular Characterization of Constipation Disease as Novel Phenotypes in CRISPR-Cas9-Generated Leptin Knockout Mice with Obesity. International Journal of Molecular Sciences, 21(24), 9464.

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Intestinal Charcoal Transit Assay

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The GI transit ratio was measured using the method described elsewhere [19 (link)]. Briefly, all mice of the subset groups were fasted for 18 h before the experiment, but were given access to water ad libitum. Each mice in the subset group was fed 0.3 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma–Aldrich Co., St. Louis, MO, USA). After 30 min of administration, the mice were euthanized with CO₂, and the GI tract was collected from the abdominal cavity. The intestinal charcoal transit ratio was calculated as follows:

C h a r c o a l t r a n s i t r a t i o (%) = [(t o t a l s m a l l i n t e s t i n e l e n g t h - t r a n s i t d i s t a n c e o f c h a r c o a l m e a l) / t o t a l s m a l l i n t e s t i n e l e n g t h)] \times 100

The total GI tract length from the stomach to the anus was also measured in duplicate.

Kim J.E., Seol A., Choi Y.J., Lee S.J., Jin Y.J., Roh Y.J., Song H.J., Hong J.T, & Hwang D.Y. (2022). Similarities and differences in constipation phenotypes between Lep knockout mice and high fat diet-induced obesity mice. PLOS ONE, 17(12), e0276445.

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Charcoal Transit Ratio Measurement in Rats

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The gastrointestinal transit ratio was measured by the method described previously [18 (link)]. Briefly, SD rats were fasted for 18 h prior to the experiment, but were allowed to consume water ad libitum. Each rat in the subset group was fed 2 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co.). After 30 min of treatment, the rat was euthanized using CO₂, and the intestinal tract was collected from the abdominal cavity. Intestinal charcoal transit ratio was calculated as follows:

C h a r c o a l t r a n s i t r a t i o (%) = [(t o t a l s m a l l i n t e s t i n e l e n g t h - t r a n s i t d i s t a n c e o f c h a r c o a l m e a l) / t o t a l s m a l l i n t e s t i n e l e n g t h)] x 100

The total intestinal length was also measured from stomach to anus in duplicate.

Kim J.E., Choi Y.J., Lee S.J., Gong J.E., Lee Y.J., Sung J.E., Jung Y.S., Lee H.S., Hong J.T, & Hwang D.Y. (2021). Antioxidant activity and laxative effects of tannin-enriched extract of Ecklonia cava in loperamide-induced constipation of SD rats. PLoS ONE, 16(2), e0246363.

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Intestinal Transit and Length Measurement

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The gastrointestinal transit ratio and intestinal length were measured as described in a previous study [47 (link)]. After fasting for 18 h (except for drinking water ad libitum) prior to the experiment, SD rats were orally administered a charcoal meal (2 mL, 3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co., St. Louis, MO, USA). Thirty minutes after administering the charcoal meal, SD rats were euthanized using CO₂, and the intestinal tract from stomach to anus was collected from the abdominal cavity. Intestinal charcoal transit ratio was calculated as follows:
In addition, the total intestinal length was measured in duplicate after taking a picture.

Kim J.E., Choi Y.J., Lee S.J., Gong J.E., Jin Y.J., Park S.H., Lee H.S., Choi Y.W., Hong J.T, & Hwang D.Y. (2021). Laxative Effects of Phlorotannins Derived from Ecklonia cava on Loperamide-Induced Constipation in SD Rats. Molecules, 26(23), 7209.

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Charcoal Meal Transit Assay for Rodent Gastrointestinal Motility

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The gastrointestinal motility was measured by the method described by Choi et al. (2014 (link)) with some modifications. Animals were fasted for 18 h prior to the experiment, but were allowed to consume water ad libitum. Briefly, SD rats were fed with 1 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co., St. Louis, MO). After 30 min of treatment, these rats were euthanized using CO₂ and the intestinal tract was collected from the abdominal cavity. The intestinal charcoal transit ratio was calculated as follows:

Charcoal transit ratio (%) = [(total small intestine length – transit distance of charcoal meal) /total small intestine length] ×100

Kim J.E., Lee M.R., Park J.J., Choi J.Y., Song B.R., Son H.J., Choi Y.W., Kim K.M., Hong J.T, & Hwang D.Y. (2018). Quercetin promotes gastrointestinal motility and mucin secretion in loperamide-induced constipation of SD rats through regulation of the mAChRs downstream signal. Pharmaceutical Biology, 56(1), 309-317.

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Antioxidant and Anti-Inflammatory Analysis

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Gum arabic, charcoal meal, red phenol, methyl cellulose, sodium hydroxide (NaOH),
NaCl, hydrochloric acid (HCl), 2,2-diphényl 1-picrylhydrazyl (DPPH),
butylhydroxyanisol (BHA), 2-thiobarbituric (TBA), 5,5′-dithiobis (2-nitrobenzoic
acid) (DTNB), trichloroacetic acid (TCA), methanol, ether, epinephrine, bovine
catalase, Folin Ciocalteu, GSH, tris, hydrogen peroxide
(H₂O₂), and yohimbine were obtained from sigma
chemicals Co (Sigma-AldrichGmbH, Steinheim, Germany). Loperamide hydrochloride
was purchased from local pharmacy and the other chemicals were used of
analytical grade.

Abidi C., Rtibi K., Boutahiri S., Tounsi H., Abdellaoui A., Wahabi S., Gressier B., Eto B, & Sebai H. (2022). Dose-dependent Action of Zingiber officinale on Colonic Dysmotility and Ex Vivo Spontaneous Intestinal Contraction Modulation. Dose-Response, 20(3), 15593258221127556.

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Charcoal Meal Transit Assay in Rats

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The gastrointestinal motility was measured by the method described by Choi et al. [51 (link)] and Kim et al. [52 (link)] with some modifications. Briefly, SD rats were fasted for 18 h prior to the experiment but were allowed to consume water ad libitum. These rats were fed with 1 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co., St. Louis, MO, USA). After 30 min of treatment, these rats were euthanized using CO₂ and the intestinal tract was collected from the abdominal cavity. The intestinal charcoal transit ratio was calculated as follows:

Kim J.E., Park J.W., Kang M.J., Choi H.J., Bae S.J., Choi Y.S., Lee Y.J., Lee H.S., Hong J.T, & Hwang D.Y. (2019). Anti-Inflammatory Response and Muscarinic Cholinergic Regulation during the Laxative Effect of Asparagus cochinchinensis in Loperamide-Induced Constipation of SD Rats. International Journal of Molecular Sciences, 20(4), 946.

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Charcoal Transit Assay in Mice

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The GI transit ratio was measured by applying the method described previously [58 (link)]. Briefly, all experimental mice were fed 1 mL of charcoal meal (3% suspension of activated charcoal in 0.5% aqueous methylcellulose) (Sigma-Aldrich Co.); 30 min after administration, the mice were euthanized using CO₂, and the intestinal tract was collected from the abdominal cavity. Intestinal charcoal transit ratio was calculated as follows:
The total intestinal length was also measured from stomach to anus, in duplicate.

Choi Y.J., Park J.W., Kim J.E., Lee S.J., Gong J.E., Jung Y.S., Seo S, & Hwang D.Y. (2021). Novel Characterization of Constipation Phenotypes in ICR Mice Orally Administrated with Polystyrene Microplastics. International Journal of Molecular Sciences, 22(11), 5845.

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Characterization of Pharmacological Compounds

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Clonidine, red phenol, NaCl, NaOH, charcoal meal, gum arabic, and haematoxylin/eosin were purchased from Sigma Chemical Co. (Sigma-Aldrich GmbH, Steinheim, Germany). Indomethacin and vinblastine were purchased from a local central pharmacy. All other reagents used were of analytical grade.

Rtibi K., Selmi S., Wannes D., Jridi M., Marzouki L, & Sebai H. (2019). The potential of Thymus vulgaris aqueous extract to protect against delayed gastric emptying and colonic constipation in rats. RSC Advances, 9(36), 20593-20602.

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