Gallocatechin 3 gallate

The chemical standards caffeic acid, p-coumaric acid, trans-caftaric acid, trans-piceid, (−)-epigallocatechin (EGC), (−)-gallocatechin (GC), and procyanidin B1 (PB1) (Phytolab, Vestenbergsgreuth, Germany); cyanidin-3-glucoside (cy-3-glc), procyanidin B2 (PB2), quercetin (Q), kaempferol (K), isorhamnetin (I), the 3-glucosides of Q, K, I and 3-galactosides of Q, K, and I (−)-catechin 3-gallate (CG), (−)-epicatechin 3-gallate (ECG), and (−)-epigallocatechin 3-gallate (EGCG) (Extrasynthese, Genay, France); gallic acid, trans-resveratrol, (+)-catechin (C), (−)-epicatechin (EC), and (−)-gallocatechin 3-gallate (GCG) (Sigma-Aldrich, Steinheim, Germany) were used. The non-commercial flavonol standard of quercetin 3-glucuronide (Q-3-glcU) was previously isolated from the skin of Petit Verdot grapes, and the Procyanidin B4 (PB4) was kindly supplied by the Department of Biochemistry and Biotechnology, Universitat Rovira i Virgili, Spain. The trans- isomers of resveratrol and its 3-glucosides (piceid) were obtained according to the methodology previously described [36 (link)]. All solvents used were chromatographic grade (>99%); the chemical standard was analytical grade (>95%) and ultrapure water (Milli-Q system) was used.

5-Azacytidine (5azaC), gallic acid (GA), (−)-gallocatechin (GC), (−)-epigallocatechin (EGC), catechin (C), caffeine (CAF), (−)-epigallocatechin gallate (EGCG), (−)-epicatechin (EC), (−)-gallocatechin-3-gallate (GCG), and (−)-epicatechin gallate (ECG) — were purchased from Sigma-Aldrich Co. USA. Anti-DNMT1 (GTX116011), anti- DNMT3A (GTX129125), and anti-DNMT3B (GTX129127) (GeneTex Co. Taiwan).