Silica gel 60 rp 18 f254s pre coated plates

All the chemicals used in the biochemical assay were purchased from Sigma-Aldrich Chemie Gmbh (Germany) and Merck (Germany) companies. The chemicals were of analytical grade. The enzyme (EC 3.2.1.1) was purchased from Sigma (Germany) that extracted from soy bean source. α-amylase activity was determined by measuring the absorbance of the mixtures at 540 nm in Elisa stat fax 2100 (Awarness Technology Inc., FL, USA). ¹H-NMR and¹³C-NMR spectra were recorded on a Brucker Avance 500 DRX spectrometer^® (Germany) with tetramethylsilane as an internal standard and chemical shifts are given in d (ppm). Multiple-pulse experiments (HSQC, HMBC, and H-H COSY) were performed using the standard Bruker^® programs. Silica gel 60 F₂₅₄ and Silica gel 60 RP-18 F₂₅₄ S pre-coated plates (Merck^®, Germany) were used for thin layer chromatography. The spots were detected by spraying with anisaldehyde-H₂SO₄ reagent (Mahestan Shimi, Iran), followed by heating.