Icc50 hd light microscope

The test isolates were subjected to conventional tests for pH, temperature, and salt (NaCl) requirements in growth media. The pH, temperature and NaCl requirements for bacterial growth were measured in Nutrient Broth (NB) media at 600 nm wavelength in spectrophotometer 13 . Firstly, NB media were prepared and, then a full loop of each test isolate on NA was transferred to broth media and incubated at different temperatures (4-35°C) for 48 hours. To measure the response to pH changes during growth, NB media were prepared and pHs of broth media were adjusted to different points in pH 3-11 range, before autoclaving. Then, a full loop of each test isolate on NA was transferred to broth media and incubated at 15°C for 48 hours. The salt (NaCl) requirement for growth was also tested in NB media containing 2-15 (w/v). To this aim, NB media were prepared and salt at predetermined concentrations was added to broth media for each isolate, before autoclaving. Then, a full loop of each test isolate on NA was transferred to broth media and incubated at 15°C for 48 hours. For each test, microbial growth was measured at 600 nm wavelength at the end of the incubation period. Cell and colony morphology (Leica ICC50 HD light microscope), Gram and endospore staining, motility and the presence of catalase and oxidase reactions were also investigated [13] [14] (link)[15] (link) .