Residual moisture content in single matrix post-desiccation was determined with a Karl Fischer moisture analyser equipped with coulometer (Metrohm), and Hydranal-Coulomat titration solution (Honeywell, Fluka) in accordance with the manufacturers’ recommendations. A standard was used to calibrate the instrument performance (lactose standard 5%, MerckMillipore). Residual moisture content was expressed as a percentage (calculated from the measured mass of water as a proportion of the calculated mass of solutes in the loaded sample).
Lactose standard 5
Manufactured by Merck Group
Sourced in France
Lactose standard 5 is a laboratory reference material used to calibrate and verify the performance of analytical instruments that measure lactose concentration. It is a pure, certified substance with a known lactose content.
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Lab products found in correlation
3 protocols using lactose standard 5
1
Residual Moisture Content Determination
2
Coulometric Karl Fischer Titration of Peptides
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KF titration was performed using a Titrando 851 titrator instrument (Metrohm, Herisau, Switzerland), coupled to a Metrohm 874 oven sample processor and operated by the Tiamo (version 2.3) software. The C3+ peptide samples (between 2 and 6 mg) were placed in the oven and the released water was transferred to the titrator cell via a stream of dried, high purity nitrogen carrier gas. The coulometric determinations were carried out with the KF titration reagents HYDRANAL™ Coulomat CG-K and Coulomat AG-Oven (VWR, Fontenay-sous-Bois, France). Water content was calculated after correction of water determined in blank samples. A lactose standard 5% (Merck, France) was used as a control sample and the SI traceability of the results was established through calibration with NIST SRM 2890 standard (NIST, MD, USA).
3
Residual Moisture Content Determination
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Residual moisture content in single matrix post-desiccation was determined with a Karl Fischer moisture analyser equipped with coulometer (Metrohm), and Hydranal-Coulomat titration solution (Honeywell, Fluka) in accordance with the manufacturers' recommendations. A standard was used to calibrate the instrument performance (lactose standard 5%, MerckMillipore). Residual moisture content was expressed as the mass extracted per 50 µL of formulation loaded into each matrix.
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