We used a 0.5 cm segment of the proximal colon for analysis. RNA was extracted using TRIZOL reagent (Life Technologies, Gaithersburg, MD) and RNeasy mini kit (QIAGEN, Valencia, CA). Total RNA (100 ng) was amplified and labeled using the Ambion whole-transcript expression kit (Life Technologies) and Affymetrix WT sense target labeling kit (Affymetrix, Santa Clara, CA). Affymetrix GeneChip Mouse Transcriptome Assay 1.0 ST arrays were hybridized with 540 ng of labeled sense DNA, washed, and stained using the FS450 Fluidic Station (Affymetrix). Fluorescent signal intensities of each stained chip were captured by the GeneChip Scanner 3000 7G System (Affymetrix).
Affymetrix wt sense target labeling kit
Manufactured by Thermo Fisher Scientific
Sourced in Canada
The Affymetrix WT Sense Target Labeling Kit is a laboratory tool designed for the preparation of labeled sense-strand targets for hybridization to Affymetrix GeneChip arrays. The kit provides reagents and protocols for the in vitro transcription and labeling of sense-strand cRNA from total RNA or purified mRNA samples.
Automatically generated - may contain errors
Lab products found in correlation
Genechip scanner 3000 7g system, by Thermo Fisher Scientific (2 mentions)
Ambion whole transcript expression kit, by Thermo Fisher Scientific (2 mentions)
Genechip mouse transcriptome assay 1.0 st arrays, by Thermo Fisher Scientific (2 mentions)
Fs450 fluidic station, by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Rneasy mini kit, by Qiagen (2 mentions)
2 protocols using affymetrix wt sense target labeling kit
1
Colonic Transcriptome Profiling via Microarray
2
Colonic Transcriptome Profiling via Microarray
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We used a 0.5 cm segment of the proximal colon for analysis. RNA was extracted using TRIZOL reagent (Life Technologies, Gaithersburg, MD) and RNeasy mini kit (QIAGEN, Valencia, CA). Total RNA (100 ng) was amplified and labeled using the Ambion whole-transcript expression kit (Life Technologies) and Affymetrix WT sense target labeling kit (Affymetrix, Santa Clara, CA). Affymetrix GeneChip Mouse Transcriptome Assay 1.0 ST arrays were hybridized with 540 ng of labeled sense DNA, washed, and stained using the FS450 Fluidic Station (Affymetrix). Fluorescent signal intensities of each stained chip were captured by the GeneChip Scanner 3000 7G System (Affymetrix).
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