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H1 human escs

Manufactured by WiCell

H1 human ESCs are a well-characterized line of human embryonic stem cells. They are derived from the inner cell mass of a human blastocyst and exhibit the key characteristics of pluripotency.

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2 protocols using h1 human escs

1

Human ESC Endothelial Differentiation and Glutamine

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H1 human ESCs (WiCell) were cultured with conditioned medium containing fresh 10ng/ml basic fibroblast growth factor (bFGF, R&D Systems) and 100ng/ml heparin (Sigma Aldrich). For spontaneous differentiation, medium was not conditioned and bFGF/heparin were left out. Endothelial differentiation was started by adding 20ng/ml bFGF, 25ng/ml bone morphogenetic protein 4 (BMP4), and 50ng/ml vascular endothelial growth factor (VEGF) (all from R&D Systems). To measure the effect of glutamine withdrawal on endothelial differentiation, glutamine was removed from the medium for the final 2 days of a 4 day differentiation.
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2

Culturing Human Cell Lines for Research

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H1 human ESCs (WiCell Research Institute) were cultured as previously described (27 (link)), on mitomycin-C-inactivated MEF feeders. Prior to transfection, H1 human ESCs were cultured feeder-free in mTeSR1 medium (Stemcell Technologies), on Matrigel (BD Biosciences). Medium was changed daily and cells were subcultured with collagenase IV (Life technologies) every three days (27 (link)). Human somatic cell lines were obtained from ATCC. LO2 and HEK293T cells were cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS); SMMC-7721, BEL-7402, BEL-7404 and H1299 cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 supplemented with 10% FBS; HK2 cells were cultured in 1:1 F-12/DMEM medium supplemented with 10% FBS; and HCT116 cells were cultured in McCoy 5A medium supplemented with 10% FBS. All media and sera were purchased from Life Technologies. All cells were incubated at 37°C and 5% CO2.
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