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Tryptone soy broth (tsb)

Manufactured by Solarbio

Tryptone soy broth is a general-purpose microbiological growth medium. It provides a nutrient-rich environment for the cultivation of a wide range of bacteria and other microorganisms.

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2 protocols using tryptone soy broth (tsb)

1

Antibacterial Hydrogel Scaffold Synthesis

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3-(aminopropyl)trimethoxysilane (Aladdin, ≥98%), rose bengal (Sigma-Aldrich, ≥80%), dopamine hydrochloride (Sigma-Aldrich, ≥99%), Tris base (Sigma-Aldrich, ≥99%), sulfhydryl-modified polyethylene glycol (PEG-SH) MW2000 (PEG2k-SH, Aladdin, ≥99%), 2-mercaptoethylamine hydrochloride (Sigma-Aldrich, ≥98%), tetracycline (Aladdin, ≥98%), d-alanine (Aladdin, ≥98%), tryptone soy broth (Solarbio Life Science, pH = 7.4), phosphate buffer (PBS, Gibco, pH = 7.4), sodium alginate (Aladdin, AR), gelatin (MW ∼50,000–100,000, type A from porcine skin), SYTO 9 (Thermo Fisher Scientific), E. coli (ATCC25922), E. coli-β (ATCC35218), P. aeruginosa (ATCC27853), S. aureus (ATCC6538), Methicillin-resistant Staphylococcus aureus (MRSA, ATCC43300) and E. faecalis (ATCC29212) were purchased from LuWei Tech. Co., Ltd., Shanghai, China.
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2

Antifungal Efficacy of Ketoconazole on Microsporum canis

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M. canis samples were taken from the skin of dogs suffering from skin diseases from an animal hospital in Shenyang, China. Subsequently, clinical samples were cultured using Sabouraud's Dextrose Agar medium (Coolaber, China), and colonies similar to the morphology of M. canis were isolated and purified by using potato dextrose agar (PDA) medium (Solarbio, China). Subsequently, Tryptone Soy Broth (Solarbio) was cultured in a fungus incubator at 28°C for 96 h, and the concentration of the fungal solution was adjusted to 2 × 104 CFU/mL. KTZ was purchased from Solarbio Ltd. (Solarbio) and dissolved in dimethyl sulfoxide (DMSO). The experiment was divided into 2 groups: the test group (T4,T5, and T6) treated with KTZ at a concentration of ½MIC for 6 h, and the control group (T1,T2, and T3) treated with DMSO at the same concentration. Tests on specimens in each group were repeated 3 times.
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