The NALC-NaOH DDM was performed using 2–3 ml of sputum in 14 ml BD Falcon tube as suggested by Kent & Kubica with few modifications (final NaOH concentration 1%) [14 ]. Briefly, 4% NaOH solution was mixed with an equal quantity of sodium citrate solution (2.9%) to prepare the working solution. Before the use of the decontamination solution, NALC powder was added to yield a final concentration of 0.5%. After the DDM procedure, processed sputum (0.2 ml) was inoculated on two slants of Löwenstein-Jensen (LJ) medium (Himedia, Mumbai, India) [one LJ slant with glycerol and the other slant with sodium pyruvate]. These slants were further incubated at 37°C aerobically and culture was monitored for growth of MTB weekly once for eight weeks.
L wenstein jensen lj medium
Manufactured by HiMedia
Sourced in India
Löwenstein-Jensen (LJ) medium is a solid culture medium used for the isolation and cultivation of Mycobacterium species, particularly Mycobacterium tuberculosis. It provides the necessary nutrients and growth factors required for the propagation of these slow-growing mycobacteria.
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Lab products found in correlation
Coumarin 6, by Merck Group (1 mentions)
Middlebrook 7h9 medium, by BD (1 mentions)
Acetonitrile and methanol, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Petri plates, by HiMedia (1 mentions)
Plga 50 50, by Durect (1 mentions)
Blood agar media, by HiMedia (1 mentions)
Dulbecco s modified eagle s medium dmem, by HiMedia (1 mentions)
2 protocols using l wenstein jensen lj medium
1
NALC-NaOH Decontamination of Sputum for MTB
2
Development of PLGA Nanoparticles for Tuberculosis
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PLGA 50:50 (MW: 1,00,000–1,20,000 Da) was procured from Durect Corporation (Birmingham, AL, USA), RIF from Duchefa-Genetix Biotech, acetonitrile and methanol from Merck Millipore (Billerica, MA, USA) and INH, benzaldehyde, ethyl alcohol and trifluoroacetic acid (TFA) from Loba Chemie (Mumbai, India). The rest of the reagents and chemicals used were of high-performance liquid chromatography (HPLC) grade. Polyvinyl alcohol (PVA) (87%–89% hydrolyzed, MW: 1,20,000 Da) was procured from Thomas Baker (Mumbai, India). Middlebrook 7H9 medium, antimicrobial mixture MGIT™ PANTA™ and MGIT™ growth supplement oleic acid-albumin-dextrose-catalase (OADC) were procured from Becton Dickinson (Franklin Lakes, NJ, USA). Löwenstein–Jensen (LJ) medium, blood agar media, Petri plates and disposable loops were procured from HiMedia (Mumbai, India). The cell culture requirements, Ham’s F-12 medium, Dulbecco’s Modified Eagle’s Medium (DMEM; HiMedia), fetal bovine serum (FBS) and Coumarin 6 were procured from Sigma-Aldrich (St. Louis, MO, USA).
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