B6 cg tg vil1 cre 1000gum j

Mice were housed in ventilated cages on a 12-hour light/12-hour dark cycle with corncob bedding and ad libitum access to rodent chow (PicoLab Rodent Diet 20, LabDiet) and water unless otherwise noted. Rbm47^fl/fl and Rbm47-IKO lines were generated on a C57BL/6J background, as described previously (7 (link)). The studies used constitutive Vil-Cre [B6.Cg-Tg(Vil1-cre)1000Gum/J], The Jackson Laboratory 021504. We also used the tamoxifen-inducible Vil-Cre [B6.Cg-Tg(Vil1-cre/ERT2)23Syr/J], The Jackson Laboratory 020282 (47 (link)), to generate inducible Rbm47-IKO mice. Because inducible and constitutive Rbm47-IKO mice exhibited similar phenotypes at baseline, further experiments were done only on the constitutive Rbm47-IKO line. Studies show that the Vil1Cre/1000 transgene is expressed extensively in small and large intestine, with only scattered expression in the urogenital tract (48 (link)). Studies were performed on chow-fed mice in the range of 8–14 weeks or where indicated in about 12-month-old mice. Where indicated, mice were fed a high–milk fat diet (TD.09766, Envigo Teklad) for 6 months prior to study. Mice received 100 mg/kg BrdU (MilliporeSigma) 2 hours before harvest. Rbm47^fl/fl mice were used as controls in all experiments.