For urine collection, animals were housed in metabolic cages and 24 hours
urine samples were collected in tubes containing thymol crystals to inhibit
bacterial growth. Samples were analyzed for creatinine (Sekisui Diagnostics,
Lexington, Massachusetts, USA), Na+, and K+ (Vitros
Chemistry, Ortho-Clinical Diagnostics, Piscataway, New Jersey, USA). Blood
samples were collected from the tail vein after fasting for 12 hours to
determine glucose (OneTouch Ultra2, LifeScan, Milpitas, California, USA),
insulin (Rat Insulin ELISA, Mercodia AB, Uppsala, Sweden), uric acid, and
creatinine levels (Vitros Chemistry, Ortho-Clinical Diagnostics).
urine samples were collected in tubes containing thymol crystals to inhibit
bacterial growth. Samples were analyzed for creatinine (Sekisui Diagnostics,
Lexington, Massachusetts, USA), Na+, and K+ (Vitros
Chemistry, Ortho-Clinical Diagnostics, Piscataway, New Jersey, USA). Blood
samples were collected from the tail vein after fasting for 12 hours to
determine glucose (OneTouch Ultra2, LifeScan, Milpitas, California, USA),
insulin (Rat Insulin ELISA, Mercodia AB, Uppsala, Sweden), uric acid, and
creatinine levels (Vitros Chemistry, Ortho-Clinical Diagnostics).