Mc38 colon carcinoma cells

Manufactured by Kerafast

The MC38 colon carcinoma cells are a cell line derived from a colon adenocarcinoma in a C57BL/6 mouse. They are commonly used in cancer research as a model for colon cancer.

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Lab products found in correlation

Glutamax, by Thermo Fisher Scientific (1 mentions) Hepes, by Thermo Fisher Scientific (1 mentions) Zeocin, by Thermo Fisher Scientific (1 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Glutamax, by Merck Group (1 mentions) Rpmi 1640, by Kerafast (1 mentions) Glutamax, by Kerafast (1 mentions) Hepes, by Kerafast (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) B16f10 melanoma cells, by American Type Culture Collection (1 mentions) Plasmotest kit, by InvivoGen (1 mentions)

Spelling variants of this product

MC38 cells (17 citations) MC38 colon (2 citations)

4 protocols using mc38 colon carcinoma cells

Engineered Cell Lines for Immunotherapies

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Chinese hamster ovary (CHO) or human embryonic kidney (HEK) cells were transfected to stably express CTLA-4 and/or OX40. The following cell lines were generated and used: CHO-CTLA-4 (0.1 × 10⁶ receptors/cell), HEK-CTLA-4 (0.8 × 10⁶ receptors/cell), CHO-CTLA-4-OX40 (0.7 × 10⁶ CTLA-4 receptors/cell; 4.8 × 10⁶ OX40 receptors/cell) and CHO-OX40 (1.1 × 10⁶ receptors/cell). Moreover, CHO-FcγRI (0.1 × 10⁶ receptors/cell) were used for crosslinking of ATOR-1015. All cell lines were cultured in RPMI-1640 with Glutamax (Gibco) supplemented with 10% fetal bovine serum (FBS, GE Healthcare), 10 mM HEPES (Gibco), zeocin (250 μg/ml) and/or geneticin (600 μg/ml, Gibco) depending on selection pressure.
MB49 bladder cancer cells (EMD Millipore) were cultured in modified DMEM (high glucose, Glutamax and sodium pyruvate) supplemented with 10% FBS. MC38 colon carcinoma cells (Kerafast) were cultured in RPMI-1640 with Glutamax supplemented with 10% FBS, 10 mM HEPES, 1 mM sodium pyruvate and 0.05 mM 2-mercaptoethanol.

Kvarnhammar A.M., Veitonmäki N., Hägerbrand K., Dahlman A., Smith K.E., Fritzell S., von Schantz L., Thagesson M., Werchau D., Smedenfors K., Johansson M., Rosén A., Åberg I., Winnerstam M., Nyblom E., Barchan K., Furebring C., Norlén P, & Ellmark P. (2019). The CTLA-4 x OX40 bispecific antibody ATOR-1015 induces anti-tumor effects through tumor-directed immune activation. Journal for Immunotherapy of Cancer, 7, 103.

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Subcutaneous Tumor Implantation in Mice

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MC38 colon carcinoma cells were purchased from Kerafast. B16‐F10 melanoma cells were purchased from ATCC. Cell lines were passaged in DMEM (Invitrogen) supplemented with 10% FCS and antibiotics. 3·5 × 10⁵ − 2 × 10⁶ MC38 cells in 100 µl PBS or 1·25 × 10⁵ B16‐F10 cells in 100 µl PBS were injected subcutaneously into the right flanks of mice, and tumours were measured with digital callipers at serial time‐points after implantation as previously described [37 ].

Whiteside S.K., Grant F.M., Gyori D.S., Conti A.G., Imianowski C.J., Kuo P., Nasrallah R., Sadiyah F., Lira S.A., Tacke F., Eil R.L., Burton O.T., Dooley J., Liston A., Okkenhaug K., Yang J, & Roychoudhuri R. (2021). CCR8 marks highly suppressive Treg cells within tumours but is dispensable for their accumulation and suppressive function. Immunology, 163(4), 512-520.

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Cell Line Maintenance for Tumor Studies

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The HEK293T cell line (female) was mycoplasma tested before the lentiviral viral production. Cells were maintained in a 5% CO₂, 95% air, humidified incubator at 37°C, in DMEM supplemented with 1X penicillin-streptomycin and 10% FBS (Sigma, F2442). MC38 colon carcinoma cells (female) were purchased from Kerafast and maintained in a 5% CO₂, 95% air, humidified incubator at 37°C in DMEM supplemented with 10% FBS, 100 U/mL penicillin, and 100 mg/mL streptomycin. MCA205 fibrosarcoma cells (unknown sex) were purchased from Millipore Sigma and maintained in a 5% CO₂, 95% air, humidified incubator at 37°C in RPMI supplemented with 10% FBS, 100 U/mL penicillin, 100 mg/mL streptomycin, 1 mM sodium pyruvate, 100 μM non-essential amino acids, and 0.055 nM 2-mercaptoethanol. MC38 and MCA205 cells utilized in in vivo experiments were under 5 passages from purchase.

Vilei E.M., Nicolet J, & Frey J. (1999). IS1634, a Novel Insertion Element Creating Long, Variable-Length Direct Repeats Which Is Specific for Mycoplasma mycoides subsp. mycoides Small-Colony Type. Journal of Bacteriology, 181(4), 1319-1323.

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Cell Line Characterization and Preparation for Murine Tumor Studies

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The murine MC38 colon carcinoma cells were purchased from Kerafast (Boston, MA). Murine C26 colon carcinoma cells were generously provided by the laboratory of Denis Guttridge (while at The Ohio State University) who obtained the cells through a Material Transfer Agreement with the NCI (Bethesda, MD) in 2001. Cultured murine C26 cells were maintained in RPMI, while LLC, and MC38 cells were maintained in Dulbecco's Modified Eagle Medium (DMEM). All media was supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin, at 37 °C in a humidified incubator with 5% CO2. For injection into mice, cells were harvested with trypsin, pelleted by centrifugation in FBS-supplemented DMEM, and then resuspended in sterile PBS at a concentration of 5 × 10⁶ cells/mL for C26 and MC38 or 10 × 10⁶ cells/mL for LLC. Prior to injection, all cell lines were confirmed negative for mycoplasma using the commercially available Plasmotest kit (Invivogen, San Diego, CA).

Madan T., Kishore U., Singh M., Strong P., Hussain E.M., Reid K.B, & Sarma P.U. (2001). Protective Role of Lung Surfactant Protein D in a Murine Model of Invasive Pulmonary Aspergillosis. Infection and Immunity, 69(4), 2728-2731.

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