Ion torrent 316 chip

Manufactured by Thermo Fisher Scientific

The Ion Torrent 316 chip is a semiconductor-based sequencing chip designed for use with the Ion Torrent sequencing platform. It is capable of generating DNA sequence data from a sample.

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Lab products found in correlation

Ion torrent onetouch system, by Thermo Fisher Scientific (2 mentions) Ion torrent personal genome machine, by Thermo Fisher Scientific (1 mentions) Ion total rna seq kit v2, by Thermo Fisher Scientific (1 mentions) Magnetic bead cleanup module, by Thermo Fisher Scientific (1 mentions) Bioanalyzer hs chip, by Agilent Technologies (1 mentions) Ion pgm template ot2 200 kit, by Thermo Fisher Scientific (1 mentions)

2 protocols using ion torrent 316 chip

HLH Target Gene Sequencing Using Ion Torrent

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After proper quantification of the libraries built for each sample, 10 patients were pooled together equally for application to an Ion Torrent 316 chip. The Ion Torrent OneTouch system (Life Technologies) and ES system (Life Technologies) were utilized for emulsion PCR and enrichment of the sequencing beads of the pooled libraries based on the instructions in the manufacturer’s protocol. Finally, an Ion Torrent Personal Genome Machine (PGM) (Life Technologies) and Ion Torrent 316 chip were used for HLH target gene sequencing. Raw data processing and VCF file generation were performed locally using the Ion Torrent platform-specific software Torrent Suite v3.4.2. The Ion Torrent online annotation platform Ion Reporter V4.4 was used for further detailed analysis of genetic variants.

Yang L., Tang Y., Xiao F., Xiong J., Shen K., Liu Y., Zhang W., Zheng L., Zhou J, & Xiao M. (2016). Hemophagocytic Lymphohistocytosis in the Chinese Han Population May Be Associated with an STXBP2 Gene Polymorphism. PLoS ONE, 11(8), e0159454.

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Small RNA Sequencing Protocol

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Small RNA-seq was performed as described previously (27 (link)). In brief, small RNAs were enriched from 2 μg of total RNA using the Magnetic bead cleanup module (Life Technologies). Libraries were then prepared using the Ion Total RNA-Seq Kit v2 (Life Technologies) and analysed via BioAnalyzer HS Chip (Agilent CA, USA). Afterwards, libraries (18 pM each) were clonally amplified by emulsion PCR in the IonTorrent OneTouch System (Life Technologies) using the Ion PGM Template OT2 200 Kit (Life Technologies) according to manufacturer′s protocol. Sequencing was performed in an IonTorrent Personal Genome Machine using an IonTorrent 316 Chip (Life Technologies).

Lemus-Diaz N., Ferreira R.R., Bohnsack K.E., Gruber J, & Bohnsack M.T. (2020). The human box C/D snoRNA U3 is a miRNA source and miR-U3 regulates expression of sortin nexin 27. Nucleic Acids Research, 48(14), 8074-8089.

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