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Psmc4

Manufactured by Fortis Life Sciences

The PSMC4 is a laboratory equipment product that serves as a core component in various scientific processes. It functions as a proteasome regulatory subunit, playing a crucial role in cellular protein degradation and regulation. The PSMC4 is a highly specialized piece of equipment designed to facilitate essential laboratory tasks, though its specific intended use should not be extrapolated upon.

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2 protocols using psmc4

1

Molecular Characterization of TDP-43 Pathology

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TDP‐43 (Cosmo Bio Co, TIP‐TD‐P09), TDP‐43 (Proteintech, 10782‐2‐AP), TDP‐43 phospho‐S409/410 (Cosmo Bio Co., Ltd, TIP‐PTD‐P02), TDP‐43 (C‐terminal; Proteintech, 12892‐1‐AP), ChAT (Merck, AB144P), GFP (UC Davis/NIH NeuroMab Facility, N86/8 and N86/38), PSMC4 (Bethyl Laboratories, A303‐850A and A303‐849A), tagRFP (Thermo Fisher Scientific, R10367), KPNA1 clone 114‐E12 (Thermo Fisher Scientific, 37‐0800), calnexin (Enzo Life Sciences, ADI‐SPA‐860‐F), HA 3F10 (Merck, 11867423001), GA 5F2 (Mackenzie et al, 2013), control IgG from mouse serum (Merck, I5381), and HCS CellMask™ Deep Red Stain (Thermo Fisher Scientific, H32721) were used.
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2

Immunoblotting and Immunofluorescence Protocols

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Primary antibodies BAG6 (Proteintech, 26417‐1‐AP), Calnexin (Abcam, ab22595), C9orf72 (GeneTex, GTX632041), C9orf72 (Proteintech, 25757‐1‐AP), GET4 (Proteintech, 27768‐1‐AP), GFP (Roche, 11814460001), HA (Cell Signaling, 3724S), NRF2 (Abcam, ab62352), PSMC4 (Bethyl Laboratories, A303‐850A), SGTA (Proteintech, 11019‐2‐AP), SMCR8 (Abcam, ab202283), α‐tubulin (Abcam, ab7291), Ubiquitin clone FK2 (Enzo Life Sciences, BML‐PW8810), Ubiquitin K11 clone 2A3/2E6 (Genentech), Ubiquitin K48 clone Apu2 (Millipore, 05‐1307), Ubiquitin K63 clone Apu3‐A8 (Genentech), Ubiquitin K11/K48‐bispecific (Genentech), UBL4A (Proteintech, 14253‐1‐AP) and UBR5 (Cell Signaling, 65344) were used for immunoblotting at a dilution of 1:1,000 in 5% milk in Tris‐buffered saline with Tween20 (TBS‐T). C9orf72 (Proteintech, 22637‐1‐AP) antibody was used for immunofluorescence staining at a dilution of 1:50 in 0.1% BSA in PBS. For secondary antibodies in immunoblotting anti‐mouse‐HRP (Promega, W402B), anti‐rabbit‐HRP (Promega, W401B), and anti‐human‐HRP (Promega, W403B) were applied at a dilution of 1:10,000. For immunofluorescence, the secondary antibody goat anti‐rabbit AlexaFluor™594 (Life Technologies, A11012) was used at a dilution of 1:1,000.
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