Immunohistochemical staining was performed according to previously described methods [26 (link)]. The following primary antibodies were used: mouse monoclonal anti-α-SMA antibody (Sigma-Aldrich), rabbit polyclonal anti-collagen type I antibody (Abcam, Cambridge, UK), rabbit polyclonal anti-collagen type III antibody (Abcam), rabbit polyclonal anti-CD3 antibody (Dako), rabbit polyclonal anti-CD68 antibody (Abcam), rabbit monoclonal anti-CD163 antibody (Abcam), and mouse monoclonal anti-EGFP antibody (Takara Bio, Shiga, Japan). CD3-, CD68-, and CD163-positive cells and positive areas for α-SMA and collagen type I and III staining were assessed using ImageJ software by examination of five randomly selected fields (× 100) of the cortex.
Rabbit polyclonal anti cd3 antibody
Manufactured by Agilent Technologies
Sourced in United Kingdom
The Rabbit polyclonal anti-CD3 antibody is a laboratory reagent used for the detection and quantification of the CD3 protein. CD3 is a marker expressed on the surface of T cells, and this antibody can be used to identify and analyze T cell populations in various research applications.
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2 protocols using rabbit polyclonal anti cd3 antibody
1
Immunohistochemistry for Tissue Analysis
2
Immunohistochemical Analysis of Tissue Markers
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Immunohistochemical staining of formalin-fixed, paraffin-embedded tissue Sects. (4 μm thick) was performed in accordance with previously described methods [25 (link)]. The following primary antibodies were used: mouse monoclonal anti-α-SMA antibody (Sigma-Aldrich), rabbit polyclonal anti-CD3 antibody (Dako), rabbit polyclonal anti-CD68 antibody (Abcam), and rabbit monoclonal anti-CD163 antibody (Abcam). CD3-, CD68-, and CD163-positive cells and positive areas for α-SMA staining were determined using ImageJ software by examination of five randomly selected fields (× 200) of the cortex.
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