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Rnascope 2.5 ls brown detection kit

Manufactured by Advanced Cell Diagnostics
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The RNAscope 2.5 LS (brown) detection kit is a laboratory equipment product designed for the detection and visualization of RNA targets in fixed tissue samples. The kit utilizes a proprietary RNA in situ hybridization technology to enable the specific and sensitive detection of target RNA molecules.

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Lab products found in correlation

Bond rx, by Leica (3 mentions) Bond rx autostainer, by Leica (1 mentions) Basescope, by Advanced Cell Diagnostics (1 mentions)

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RNAscope kit (59 citations)

4 protocols using rnascope 2.5 ls brown detection kit

1

In situ Hybridization of Stem Cell Markers

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In situ hybridisation detection for Lgr5 (312178), Olfm4 (311838), Axin2 (400338), Bcl9 (529268) and Bcl9l (466698) mRNA (All Advanced Cell Diagnostics) was performed using RNAscope 2.5 LS (Brown) Detection Kit (Advanced Cell Diagnostics) on a Bond Rx autostainer (Leica) strictly according to the manufacturer’s instructions. Basescope (Advanced Cell Diagnostics) ApcEx14 #701641 (detects wild-type Apc exon 14) was used according to the manufacturer’s instructions.
Gay D.M., Ridgway R.A., Müller M., Hodder M.C., Hedley A., Clark W., Leach J.D., Jackstadt R., Nixon C., Huels D.J., Campbell A.D., Bird T.G, & Sansom O.J. (2019). Loss of BCL9/9l suppresses Wnt driven tumourigenesis in models that recapitulate human cancer. Nature Communications, 10, 723.
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2

In Situ Hybridization Analysis of Intestinal Stem Cell Markers

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In situ hybridization analysis was performed by using RNAscope 2.5 LS (Brown) detection kit (Advanced Cell Diagnostics) on a Leica Bond Rx autostainer according to the manufacturer’s instructions. Detection of the expression of Axin2 and Lgr5 was analyzed. Positive staining was identified as brown, punctate dots. Crypts were encircled manually and the positive staining area for each crypt was measured via Fiji. A threshold was set to mark the area of positive staining only. This threshold was maintained for the analysis of the entire image. Intestinal sections were counterstained with hematoxylin to visualize the nuclei. Ppib was used as a positive control.
Walter R.J., Sonnentag S.J., Munoz-Sagredo L., Merkel M., Richert L., Bunert F., Heneka Y.M., Loustau T., Hodder M., Ridgway R.A., Sansom O.J., Mely Y., Rothbauer U., Schmitt M, & Orian-Rousseau V. (2022). Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop. Cell Death & Disease, 13(2), 168.
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3

In Situ Hybridization for WNT7A and PPIB

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In situ hybridization staining was performed on 4um formalin fixed paraffin embedded sections which had previously been ovened at 600C for 2 hours. In situ hybridization detection for WNT7a (401128) and PPIB (313918) (Advanced Cell Diagnostics, Hayward, CA) mRNA was performed using RNAscope 2.5 LS (brown) detection kit (322100; Advanced Cell Diagnostics, Hayward, CA) and performed on a Leica Bond Rx autostainer strictly adhering to the manufacturer’s instructions. WNT7A RNAscope analysis is provided in Table S7.
Brunton H., Caligiuri G., Cunningham R., Upstill-Goddard R., Bailey U.M., Garner I.M., Nourse C., Dreyer S., Jones M., Moran-Jones K., Wright D.W., Paulus-Hock V., Nixon C., Thomson G., Jamieson N.B., McGregor G.A., Evers L., McKay C.J., Gulati A., Brough R., Bajrami I., Pettitt S.J., Dziubinski M.L., Barry S.T., Grützmann R., Brown R., Curry E., Pajic M., Musgrove E.A., Petersen G.M., Shanks E., Ashworth A., Crawford H.C., Simeone D.M., Froeling F.E., Lord C.J., Mukhopadhyay D., Pilarsky C., Grimmond S.E., Morton J.P., Sansom O.J., Chang D.K., Bailey P.J, & Biankin A.V. (2020). HNF4A and GATA6 Loss Reveals Therapeutically Actionable Subtypes in Pancreatic Cancer. Cell reports, 31(6), 107625.
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4

In Situ Hybridization for WNT7A and PPIB

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In situ hybridization staining was performed on 4um formalin fixed paraffin embedded sections which had previously been ovened at 600C for 2 hours. In situ hybridization detection for WNT7a (401128) and PPIB (313918) (Advanced Cell Diagnostics, Hayward, CA) mRNA was performed using RNAscope 2.5 LS (brown) detection kit (322100; Advanced Cell Diagnostics, Hayward, CA) and performed on a Leica Bond Rx autostainer strictly adhering to the manufacturer’s instructions. WNT7A RNAscope analysis is provided in Table S7.
Brunton H., Caligiuri G., Cunningham R., Upstill-Goddard R., Bailey U.M., Garner I.M., Nourse C., Dreyer S., Jones M., Moran-Jones K., Wright D.W., Paulus-Hock V., Nixon C., Thomson G., Jamieson N.B., McGregor G.A., Evers L., McKay C.J., Gulati A., Brough R., Bajrami I., Pettitt S.J., Dziubinski M.L., Barry S.T., Grützmann R., Brown R., Curry E., Pajic M., Musgrove E.A., Petersen G.M., Shanks E., Ashworth A., Crawford H.C., Simeone D.M., Froeling F.E., Lord C.J., Mukhopadhyay D., Pilarsky C., Grimmond S.E., Morton J.P., Sansom O.J., Chang D.K., Bailey P.J, & Biankin A.V. (2020). HNF4A and GATA6 Loss Reveals Therapeutically Actionable Subtypes in Pancreatic Cancer. Cell reports, 31(6), 107625.
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