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Mouse lif

Manufactured by Miltenyi Biotec
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Mouse LIF is a recombinant protein that functions as a cytokine. It maintains the self-renewal and pluripotency of mouse embryonic stem cells.

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Lab products found in correlation

Human tgf β1, by R&D Systems (2 mentions) Recombinant human lif, by R&D Systems (2 mentions) Human oncostatin m, by R&D Systems (2 mentions) Mouse oncostatin m, by R&D Systems (2 mentions)

2 protocols using mouse lif

1

Cytokine Reconstitution and Cell Treatment

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Recombinant human LIF (R&D Systems), human oncostatin M (R&D Systems), mouse LIF (Miltenyi Biotec), mouse oncostatin M (R&D Systems), and human TGF-β1 (R&D Systems) were reconstituted in PBS + 0.1% bovine serum albumin (BSA) at 10–25μg/ml and aliquoted for storage at −80°C. For all experiments, mouse recombinant proteins were used on mouse cell lines, and human recombinant proteins were used on human cell lines, with the exception of human TGF-β1, which was used on all cell lines since TGF-β1 maintains approximately 99% sequence homology between human and mouse species63 (link). Prior to cytokine treatment cells were serum starved in 2% FBS overnight and cytokine treatment was made up in media containing 2% FBS.
Johnson R.W., Finger E.C., Olcina M.M., Vilalta M., Aguilera T., Miao Y., Merkel A.R., Johnson J.R., Sterling J.A., Wu J.Y, & Giaccia A.J. (2016). Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow. Nature cell biology, 18(10), 1078-1089.
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2

Cytokine Reconstitution and Cell Treatment

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human LIF (R&D Systems), human oncostatin M (R&D Systems), mouse LIF (Miltenyi Biotec), mouse oncostatin M (R&D Systems), and human TGF-β1 (R&D Systems) were reconstituted in PBS + 0.1% bovine serum albumin (BSA) at 10–25μg/ml and aliquoted for storage at −80°C. For all experiments, mouse recombinant proteins were used on mouse cell lines, and human recombinant proteins were used on human cell lines, with the exception of human TGF-β1, which was used on all cell lines since TGF-β1 maintains approximately 99% sequence homology between human and mouse species63 (link). Prior to cytokine treatment cells were serum starved in 2% FBS overnight and cytokine treatment was made up in media containing 2% FBS.
Johnson R.W., Finger E.C., Olcina M.M., Vilalta M., Aguilera T., Miao Y., Merkel A.R., Johnson J.R., Sterling J.A., Wu J.Y, & Giaccia A.J. (2016). Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow. Nature cell biology, 18(10), 1078-1089.
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