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Fitc conjugated mouse igg1 isotype control

Manufactured by Thermo Fisher Scientific
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FITC-conjugated mouse IgG1 Isotype Control is a fluorescently labeled non-specific control antibody used to establish baseline fluorescence levels in flow cytometry experiments. It serves as a reference to differentiate specific from non-specific antibody binding.

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Lab products found in correlation

Cd3 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti mouse igg fitc, by Thermo Fisher Scientific (1 mentions) Cd3 pecy5, by Thermo Fisher Scientific (1 mentions) Mouse igg1 isotype control, by Thermo Fisher Scientific (1 mentions) Rabbit igg isotype control, by Abcam (1 mentions) Fitc conjugated annexin 5, by Beckman Coulter (1 mentions) Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Mouse IgG1 isotype control IgG1 isotype control FITC-conjugated mouse IgG1 Mouse IgG1-FITC Isotype controls IgG1 FITC Mouse IgG1

2 protocols using fitc conjugated mouse igg1 isotype control

1

Flow Cytometry Antibody Staining Protocol

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Mouse anti-human CD35 monoclonal IgG1 antibody (clone 3D9) was prepared as described [17 (link)]. FITC-conjugated mouse IgG1 Isotype Control (eBioscience, Frankfurt, Germany) was used for FACS experiments. Anti-human CD35-FITC (clone 3D9, kindly provided by Dr. J. Prechl, Diagnosticum zrt, Budapest, Hungary), anti-human CD19-eFlour450, CD3-PeCy5, CD3-PeCy7 (eBioscience), and anti-mouse IgG-FITC (Invitrogen, Waltham, Massachusetts, USA) antibodies were used for FACS experiments. Mouse IgG1 kappa-eFluor 450 and IgG1 kappa-PE-Cy7 Isotype Controls (eBioscience) were used also for FACS experiments.
Ralchev N.R., Markovski A.M., Yankova I.A., Manoylov I.K., Doytchinova I.A., Mihaylova N.M., Shinkov A.D, & Tchorbanov A.I. (2022). Selective Silencing of Disease-Associated B Lymphocytes from Hashimoto’s Thyroiditis Patients by Chimeric Protein Molecules. International Journal of Molecular Sciences, 23(23), 15083.
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2

Antibody Panel for Alzheimer's Biomarkers

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The following antibodies were employed: rabbit anti-Aβ1-42 monoclonal antibody (clone H31L21, Invitrogen, Carlsbad, CA), which recognizes human and mouse Aβ1-42; cross reactivity to Aβ1-40 is not observed in sandwich ELISA according to the manufacturer’s instructions; mouse anti-human Aβ monoclonal antibody (clone 6E10, Covance, Princeton, NJ), mouse anti-human apoE monoclonal antibody (clone 1H4, Abcam, Cambridge, MA); mouse anti-human apoJ monoclonal antibody (clone 3R3/2, Lifespan Biosciences, Seattle, WA); rabbit anti-mouse apoJ polyclonal antibody (Lifespan Biosciences); fluorescein isothiocyanate (FITC)-conjugated mouse anti-human apoAI monoclonal antibody (clone APO-1-1, Fitzgerald Industries, Acton, MA); rabbit anti-mouse apoAI polyclonal antibody (Lifespan Biosciences); FITC-conjugated annexin V (Beckman Coulter, Pasadena, CA); and mouse anti-human tau monoclonal antibody (clone TAU-5, Abcam). Isotype controls were: FITC-conjugated mouse IgG1 isotype control (eBioscience, San Diego, CA); mouse IgG1 isotype control (eBioscience), rabbit IgG isotype control (Abcam). Other reagents included Calibration BeadMix (Apogee Flow Systems, Hemel Hempstead, UK), annexin V binding buffer 10× (Beckman Coulter), CountBright absolute counting beads (Invitrogen), Zenon Alexa Fluor 488 mouse IgG1 and phycoerythrin (PE) rabbit IgG labeling kits (Invitrogen).
Yang Y., Keene C.D., Peskind E.R., Galasko D.R., Hu S.C., Cudaback E., Wilson A.M., Li G., Yu C.E., Montine K.S., Zhang J., Baird G.S., Hyman B.T, & Montine T.J. (2015). Cerebrospinal Fluid Particles in Alzheimer Disease and Parkinson Disease. Journal of neuropathology and experimental neurology, 74(7), 672-687.
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