Fasting blood samples were collected and transferred immediately to a non-heparinized tube for centrifugation. Collected sera were then transferred into a pre-labeled plain tube, stored on ice and delivered to the Biomarkers Research Program (BRP) laboratory, King Saud University, Riyadh, Saudi Arabia, on the same day of collection for immediate storage in a −20 °C freezer for immediate analyses. The blood samples were analyzed for fasting glucose and lipid profile including HDL-cholesterol, using a chemical analyzer (Konelab, Espoo, Finland). Serum SHBG was measured with a Roche Elecsys modular analytics Cobas e411 using an electrochemiluminescence immunoassay [ECLIA] (Roche Diagnostics, GmbH, Mannheim, Germany). The lower detection limit of this assay was 0.35 nmol/L and the intra-assay CV is 2.6–5.6 %.
Elecsys modular analytics cobas e411
Manufactured by Roche
Sourced in Germany
The Elecsys modular analytics Cobas e411 is a clinical chemistry analyzer designed for in-vitro diagnostic testing. It is capable of performing various immunoassay tests, including those for hormones, proteins, and other analytes. The Cobas e411 is part of the Roche Diagnostics Elecsys modular analytics system.
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Lab products found in correlation
7 protocols using elecsys modular analytics cobas e411
1
Fasting Blood Samples Analysis
2
Anthropometric and Metabolic Assessments
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Anthropometrics which were determined included height (rounded off to the nearest 0.5 cm), weight (rounded off to the nearest 0.1 kg), waist and hip circumference (centimeters), and mean blood pressure (systolic and diastolic in mmHg) (average of two readings). Body mass index (BMI) was calculated as weight in kilograms divided by height in square meters. Fasting blood samples were collected and transferred immediately to a non-heparinized tube for centrifugation. Fasting glucose, lipid profile, were measured using a chemical analyzer (Konelab, Espoo, Finland). Serum 25(OH)D was measured by using commercially available kits using Roche Elecsys Modular Analytics Cobas e411 utilizing electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany). Serum levels of SAP and CRP were measured using ELISA kits (Abcam®, Cambridge, UK and R & D SYSTEMS®, Minneapolis, MN, USA, respectively) following manufacturers’ instructions. To minimize inter-assay variability, all samples were analyzed simultaneously and the actual variations were well within the inter- and intra-assay ranges. All measurements were done at baseline and post-intervention.
3
Serum Biomarker Measurement Protocol
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Fasting glucose, lipid profile, calcium, and phosphorous were measured using a chemical analyzer (Konelab, Espoo, Finland). Serum 25(OH)D was measured with a Roche Elecsys modular analytics Cobas e411 using an electrochemiluminescence immuno assay (Roche Diagnostics, GmbH, Mannheim, Germany) and commercially available IDS kits (IDS Ltd, Boldon Colliery, Tyne & Wear, UK). The inter- and intra-assay coefficients of variation (CV) for 25 (OH) D ELISA were 5.3% and 4.6%, respectively, with 100% cross-reactivity to 25 (OH) D3 and 75% cross-reactivity to 25 (OH) D2. It should be noted that the BRP laboratory is a participating entity in the Vitamin D External Quality Assessment Scheme (DEQAS), and Quality Assurance (QA) standards are maintained by ISO 9000 and 17025, whereas the QA department audits the BRP laboratory at regular intervals.
4
Biochemical Markers in Health Assessment
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Fasting glucose, lipid profile, calcium, and phosphorous were measured using a chemical analyzer (Konelab, Espoo, Finland). Serum 25(OH)D was measured with a Roche Elecsys modular analytics Cobas e411 using an electrochemiluminescence immunoassay (Roche Diagnostics, GmbH, Mannheim, Germany) and commercially available IDS kits (IDS Ltd, Boldon Colliery, Tyne & Wear, UK). Variation for the 25(OH)D ELISA were 5.3 % and 4.6 %, respectively, with 100 % cross-reactivity to 25(OH)D3 and 75 % cross-reactivity to 25(OH)D2. It should be noted that the BRP laboratory is a participating entity in the Vitamin D External Quality Assessment Scheme (DEQAS), and Quality Assurance (QA) standards are maintained by ISO 9000 and 17025. The QA department audits the BRP laboratory at regular intervals. Serum levels (in mmol/L) of glucose <6.1, total cholesterol <5.0, triglycerides <1.7 and calcium 2.25-2.75 were considered normal/desirable.
5
Serum Vitamin D and Calcium Assessment
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Serum samples for measuring biochemical parameters of calcium and vitamin D were obtained from all subjects; plain red vacutainer tubes were used. Then, they were stored at −20°C until the time of analysis. The 25-hydroxyvitamin D [25(OH)D] was the preferred metabolite that has been used to assess serum vitamin D levels.[27 (link)] The level of serum 25(OH)D was determined automatically as described previously using a Roche Elecsys modular analytics (Cobas e411) to perform an electrochemiluminescence immunoassay (Roche Diagnostics, GmbH, Mannheim, Germany).[28 (link)] Serum calcium was measured on an Olympus AU 400 chemistry autoanalyzer using a photometric color (Arsenazo method) test. Both analyses were performed in the Biochemistry laboratory at King Fahad General Hospital, Jeddah, KSA.
6
Serum Biomarkers Profiling Protocol
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All analyses were performed at the CBCD laboratory, KSU, Riyadh, KSA. Lipid profile and fasting glucose levels were measured by the chemical analyzer (Konelab, Espoo, Finland). Serum 25(OH)D was measured with a Roche Elecsys modular analytics Cobas e411 using an electro-chemiluminescence immunoassay (Roche Diagnostics, GmbH, Mannheim, Germany). CBCD laboratory is participating in the Vitamin D External Quality Assessment Scheme (DEQAS) and quality assurance standards are according to ISO-17025. Vitamin D binding protein (VDBP), sclerostin (SOST) and all the other biochemical parameters were measured by following the protocol and instructions mentioned in our previous paper [23 (link)].
7
Hormonal Biomarker Assessment Protocol
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Levels of FSH, LH, and AMH were assessed from fasting samples in women at the three assessment clinics without restrictions on which day in the menstrual cycle the participants were at the time of blood sampling. Women were instructed to fast overnight or for at least 8 h before the clinic visit, and the blood samples were processed within 4 h and stored at − 80 °C until thawed for hormonal analyses (with no previous thaw-freeze cycles). Serum FSH, LH and AMH were measured with a Roche Elecsys modular analytics Cobas e411 using an electrochemiluminescence immunoassay. The AMH assay used was the fully automated Elecsys AMH Plus immunoassay from Roche Diagnostics [20 (link)].
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