Blood samples were collected either in tubes containing ethylenediaminetetraacetic acid (EDTA) as an anticoagulant agent for the preparation of blood smears or in normal tubes without EDTA for serum separation and subsequent biochemical analyses. Blood samples were obtained from the brachial vein punctured by a lancet and smears were prepared on clean microscopic slides, fixed with absolute methanol, and then stained with 10% aqueous Giemsa stain for 45 min. Five hundred RBCs were counted for each animal at 4-day intervals for 16 d, to calculate the parasitemia rate after each treatment [21 (link)]. Serum was separated by centrifugation at 1800 × g for 10 min and stored at −20°C until analysis. Bilirubin, albumin, glucose, AST, and ALT concentrations were measured by commercial kits (United Diagnostic Industry, Dammam, Saudi Arabia) using a BAS 3000 semi-automatic biochemistry analyzer (Labomed, Los Angeles, CA, U.S.A.) and an Ultrospec 2100® UV-visible spectrophotometer (Biochrom, Holliston, MA, U.S.A.).
Ultrospec 2100 uv visible spectrophotometer
Manufactured by Harvard Bioscience
Sourced in United States
The Ultrospec 2100® is a UV-visible spectrophotometer designed for accurate and reliable absorbance measurements. It features a wavelength range of 190 to 900 nm and can be used to quantify a variety of samples, including proteins, nucleic acids, and other biomolecules.
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Lab products found in correlation
3 protocols using ultrospec 2100 uv visible spectrophotometer
1
Blood Sample Collection and Analysis
2
Bacterial Culture Preparation for Antimicrobial Assays
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A single colony of bacteria from a plate previously streaked from frozen stock was grown overnight in medium. MRSA substrain COL and P. aeruginosa (strain ATCC 27,853) were grown in tryptic soy broth supplemented with 1% glucose (w/v, %) (TSBG) medium and E. coli (strain UTI89) was grown in Luria-Bertani (LB) medium at 37°C. To make the working bacteria culture for the instant kill and continuous kill experiment, 100 μL of the overnight culture was added to 2 mL of medium and allowed to grow until it reached an optical density at 600 nm (OD600nm) of 0.5 OD measured with a spectrophotometer (Biochrom ULTROSPEC 2100 UV-visible spectrophotometer). For the bacteria adhesion and growth experiment, 100 μL of the overnight culture was added to 2 mL and grown to 0.5 OD600nm at 37°C, and then diluted to 0.02 OD600nm.
3
Quantifying Ozone and Microplastics
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Ultrospec 2100 UV-Visible Spectrophotometer (Biochrom, USA) was used to measure the UV absorption and standardize the O3 stock solutions. The Indigo method [Bader and Hoigné, 1981] was also employed for dissolved O3 measurements. MPs were detected using an Infinity Series HPLC of Agilent coupled with UV/Vis detector. A Zorbax Eclipse XBD C18 (150 × 4.6 mm i.d; 5 μm particle size) was the employed column.
Acetonitrile (A) and Milli-Q water adjusted to pH = 3 by orthophosphoric acid (B) were employed as mobile phases. The analyses were performed under a gradient method as follows: 30% A and 70% B initially kept for 5 min, 30% A to 60% A during 5 min, 60%
A and 40% B kept for 25 min, 60% A to 80% A during 5 min, 80% A and 20% B kept for 30 min, 80% A to 30% A during 10 min and finally 30% A and 70% B kept for 10 min. Total organic carbon (TOC) was determined using a TOC-V CNS Total Organic Carbon analyzer by Shimadzu (Japan).
Acetonitrile (A) and Milli-Q water adjusted to pH = 3 by orthophosphoric acid (B) were employed as mobile phases. The analyses were performed under a gradient method as follows: 30% A and 70% B initially kept for 5 min, 30% A to 60% A during 5 min, 60%
A and 40% B kept for 25 min, 60% A to 80% A during 5 min, 80% A and 20% B kept for 30 min, 80% A to 30% A during 10 min and finally 30% A and 70% B kept for 10 min. Total organic carbon (TOC) was determined using a TOC-V CNS Total Organic Carbon analyzer by Shimadzu (Japan).
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