Ham s f12 dulbecco s modified eagle s medium dmem

Four human osteosarcoma cell lines (MNNG/HOS, MG63, SaOS-2 and U2OS) and normal human osteoblasts cell line (hFOB 1.19) were obtained from the Cell Bank of China Academy of Sciences (Shanghai, China). MNNG/HOS, MG63 and U2OS cells were cultured in α-MEM medium (HyClone, USA) supplemented with 10% FBS (Gibco, USA). SaOS-2 cells were cultured in RPMI-1640 medium (Gibco, USA) supplemented with 15% FBS. hFOB1.19 cells were cultured in Ham’s F12/Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin (Invitrogen, Grand Island, NY, USA). Four human osteosarcoma cell lines were maintained in a humidified incubator at 37 °C with a 5% CO2 atmosphere. hFOB 1.19 cell line were maintained at 33.5 °C with a 5% CO2 atmosphere.

The cell bank of the Chinese Academy of Sciences (Shanghai, China) provided the OS cell lines (Saos2, MG63, U2OS and HOS) and a human normal osteoblast cell line hFOB1.19. MG63, HOS and U2OS cells were cultured in alpha minimal essential medium (α‐MEM; HyClone, Logan, UT, USA) supplemented with 10% foetal bovine serum (FBS; Gibco, Carlsbad, CA, USA). Saos2 cells were cultured in Rosewell Park Memorial Institute 1640 (RPMI‐1640) medium (Gibco, USA) supplemented with 15% FBS. hFOB1.19 cells were cultured in Ham's F12/Dulbecco's modified Eagle's medium (DMEM, Gibco) supplemented with 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin (Invitrogen, Grand Island, NY, USA). All four human OS cell lines were maintained in a humidified incubator at 37°C with a 5% CO₂. The hFOB 1.19 cell line was maintained at 33.5°C with a 5% CO₂.