UA was purchased from the National Institute for the Control of Pharmaceutical and Biological Products (Beijing, China). Human breast cancer MCF-7 cells were obtained from Shanghai Cell Bank of Chinese Academy of Science (Shanghai, China). Trizol was from Invitrogen (CA, USA). Microarray analysis was finished by Oebiotech (Shanghai, China). Specific antibodies for GAPDH, BRAF, PLK1, IKKβ, NF-κB p65, ERK1/2, p-ERK1/2, p-IKKβ, p-BRAF, p-NF-κB p65, and p-PLK1 were purchased from Abcam (Cambridge, UK).
Mcf 7 cells
Manufactured by Shanghai Cell Bank
Sourced in China
MCF-7 cells are an adherent cell line derived from a human breast adenocarcinoma. They are widely used in cancer research as a model for studying the biology of breast cancer cells.
Automatically generated - may contain errors
Lab products found in correlation
Hek293t cells, by Shanghai Cell Bank (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Gapdh, by Abcam (1 mentions)
P ikkβ, by Abcam (1 mentions)
P plk1, by Abcam (1 mentions)
P b raf, by Abcam (1 mentions)
Nf κb p65, by Abcam (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
P nf κb p65, by Abcam (1 mentions)
P erk1 2, by Abcam (1 mentions)
Hek293t gfp cells, by Shanghai Cell Bank (1 mentions)
Mcf 7, by Shanghai Cell Bank (1 mentions)
Hela cells, by Shanghai Cell Bank (1 mentions)
Skov3, by Shanghai Cell Bank (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Insulin, by Thermo Fisher Scientific (1 mentions)
Mcf 7 cells, by Thermo Fisher Scientific (1 mentions)
Mcf 10a, by American Type Culture Collection (1 mentions)
7 protocols using mcf 7 cells
1
Molecular Insights into Breast Cancer Progression
2
In Vitro and In Vivo Cell Culture Protocols
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HEK293T cells (human embryonic kidney transformed cells), HEK293T‐GFP cells (HEK293T cells encoding the GFP gene), MCF‐7 cells (human breast carcinoma cells), and Huh7‐Luc cells (human liver carcinoma cells encoding the luciferase gene) were purchased from the Shanghai Cell Bank, Chinese Academy of Sciences. All cells were kept at 37 °C in a humidified atmosphere containing 5% CO2 and cultivated in Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum, 1% penicillin, and 1% streptomycin sulfate. All animal experiments were performed in accordance with the regulations of the Institutional Animal Care and Use Committee (IACUC) of Zhejiang University. Nude mice (5 weeks old, 18 ± 2 g) were raised under aseptic conditions in animal isolators with free access to food and water under a 12 h light/dark cycle. The animals were observed daily during the experiment.
3
Cell Line Cultivation Protocol
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The following cells lines were purchased
from the Shanghai Cell Bank of the Chinese Academy of Sciences: HEK
293T cells (human embryonic kidney transformed cells), MCF 10A cells
(human normal mammary epithelial cells), MCF-7 cells (human breast
cancer cell), MCF-7 (PDX) cells (human breast cancer cell), SKOV3
(human ovarian cancer cell), and HeLa cells (human cervical cancer
cells). Live cells were kept at 37 °C in a humidified atmosphere
containing 5% CO2 and cultivated in Dulbecco’s modified
Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 1%
penicillin, and 1% streptomycin sulfate. MCF 10A cells were cultivated
in DMEM/F12 containing 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL
hydrocortisone, 10 μg/mL insulin, 1% NEAA, and 1% P/S.
from the Shanghai Cell Bank of the Chinese Academy of Sciences: HEK
293T cells (human embryonic kidney transformed cells), MCF 10A cells
(human normal mammary epithelial cells), MCF-7 cells (human breast
cancer cell), MCF-7 (PDX) cells (human breast cancer cell), SKOV3
(human ovarian cancer cell), and HeLa cells (human cervical cancer
cells). Live cells were kept at 37 °C in a humidified atmosphere
containing 5% CO2 and cultivated in Dulbecco’s modified
Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS), 1%
penicillin, and 1% streptomycin sulfate. MCF 10A cells were cultivated
in DMEM/F12 containing 5% horse serum, 20 ng/mL EGF, 0.5 μg/mL
hydrocortisone, 10 μg/mL insulin, 1% NEAA, and 1% P/S.
4
Breast Cancer Tissue Sample Collection
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A total of 30 primary BC tissues and 30 corresponding noncancerous tissues (>2 cm from BC tissues) were collected from primary BC patients who underwent tumors surgical resection. Ten normal control (NC) breast tissues were collected from age- and gender-matched patients who underwent mammoplasty. This study obtained the approval of the Ethics Committee of the Huaihe Hospital of Henan University and written informed consent from each patient. MCF-7 cells were purchased from Shanghai Cell Bank of Chinese Academy of Sciences (China). The cells were cultured in RPMI-1640 medium (Gibco, USA) containing 10% fetal bovine serum (FBS; Gibco).
5
MCF-7 Cell Culture Protocol
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MCF‐7 cells were purchased from Shanghai Cell Bank, Chinese Academy of Sciences. MCF‐7 cells were cultured in 5% CO2, for 24 hr at 37℃ in an incubator (Thermo). The growing medium consisted of the following: Roswell park memorial institute (RPMI) 1640 medium (HyClone), 10% Fetal bovine serum (Gibco), 2% antibiotics [penicillin in a concentration of 100 U/mL and streptomycin in a concentration of 100 U/mL (Gibco)], and 1% insulin (Gibco).
6
Cultivation of MCF-7, MCF-10A, and MCF-7/TAX Cells
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MCF-7 cells were purchased from the Shanghai Cell Bank of Chinese Academy of Sciences; MCF-10A and MCF-7/TAX cells were purchased from the American Type Culture Collection (Manassas, VA, USA). MCF-7 and MCF-10A cells were cultured in RPMI 1640 medium containing 10% fetal bovine serum. MCF-7/TAX cells were cultured in RPMI 1640 medium containing 1.0 µM paclitaxel to maintain their resistant phenotype. The cells were cultured in a humidified incubator at 37 °C in an atmosphere of 5% CO2.
7
Cell Culture Protocol for Breast and Endothelial Cells
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Human breast cell lines (MCF-10 cells), Human breast cancer cell lines (MDA-MB-231 cells, MCF-7 cells) and Human umbilical vein endothelial cells (HUVECs) were purchased from Shanghai Cell Bank of Chinese Academy of Sciences. These cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Invitrogen; Gibco; 12800017) supplemented with 10% FBS (Gibco; 16000-044) and 1% penicillin–streptomycin (Hyclone; SV30010). Cells were incubated in a humidified atmosphere at 37 °C with 5% CO2.
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