All nanotubes were weighed and suspended in dispersion media (DM) (Porter et al., 2008 ), which consisted of mouse serum albumin (Sigma, St. Louis, MO; 1 mg/mL or 0.1%) and 1,2 dipalmitoyl-sn-glycero-3-phosphocholine (DSPC, Sigma, 1 μg/mL or 0.0001%) diluted in phosphate-buffered saline (PBS). Nanotube suspensions were sonicated for 5 min at 1/3 max power in a Qsonica cup-horn sonicator (Q500, Newtown, CT) attached to a circulating water-bath (VWR International, Radnor, PA) at 500 watts and 20 Hz (8000 Joules) at a stock concentration of 1 mg/mL.
Circulating water bath
Manufactured by Avantor
The Circulating water-bath is a laboratory equipment used to maintain a constant temperature in a water-filled container. It circulates the water to ensure uniform temperature distribution. The device is designed to provide precise temperature control for various laboratory applications.
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Lab products found in correlation
4 protocols using circulating water bath
1
Nanotube Dispersion Protocol for Research
2
Jejunal Glucose Transport Dynamics
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Jejunal integrity and nutrient transport were measured using modified Ussing chambers as previously described (Saddoris et al., 2019 (link); Kpodo et al., 2020 (link)). Briefly, the outer serosal layer was removed and the remaining tissue (epithelial and submucosal layer) was mounted in modified Ussing chambers equipped to measure transepithelial resistance (TER) as well as glucose baseline Isc (bIsc ), maximum Isc (mIsc ), and the change in bIsc to mIsc (dIsc ). Tissues (1.0 cm2 surface area) were mounted in duplicates and the chambers were filled with 8 mL modified Krebs buffer solution. Tissues were kept at 37.0°C using a circulating water bath (VWR, Batavia, IL) and were continuously aerated using carbogen gas (95% O2 and 5% CO2). After tissues were allowed to equilibrate for 30 min, glucose (10 mmol/L) was added to the mucosal side and mannitol (10 mmol/L) to the serosal side (10 mmol/L) for osmotic balance. The active transport of glucose was determined by measuring the dIsc.
3
UV-Vis Spectrophotometry with Controlled Conditions
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An Ocean Optics SD2000 fiber optic UV-VIS spectrophotometer with a DT-Mini 26 s deuterium tungsten light source was used to perform all measurements. A quartz cuvette with a 1.0 cm pathlength was used for all measurements. The cuvette holder for this instrument was magnetically stirred at 300 rpm using a magnetic stirrer equipped with a digital tachometer (VWR Scientific). The temperature of the cuvette was maintained at 25 °C with a circulating water bath (VWR Scientific).
4
Nanoparticle Suspension Preparation Protocol
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