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Caspase activity assay kit

Manufactured by Abcam
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Sourced in United States

The Caspase activity assay kit is a laboratory tool used to measure the activity of caspase enzymes, which play a crucial role in the process of apoptosis, or programmed cell death. The kit provides a standardized method for quantifying the enzymatic activity of caspases in cell lysates or purified samples.

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Lab products found in correlation

M200 pro nanoquantautoreader, by Tecan (1 mentions)

Close spelling variants of this product

Caspase-3 Activity Assay Kit (44 citations) Caspase-1 activity assay kit (5 citations) Generic caspase activity assay kit (4 citations) Caspase activity kit (3 citations) Activity assay kits (2 citations) Caspase family activity assay kits (2 citations) Caspase-3 and caspase-9 activity assay kits (2 citations) Caspase 9 Multiplex Activity Assay Kit (Fluorometric) (2 citations) Caspase-3 Activity Assay Kit (Fluorometric) (2 citations) Colorimetric caspase 3 activity assay kit (2 citations)

3 protocols using caspase activity assay kit

1

Caspase 1 Activity Assay

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Caspase 1 activity was determined using a Caspase Activity Assay Kit (Abcam) according to the manufacturer’s instructions.
Chang K.H., Lee-Chen G.J., Huang C.C., Lin J.L., Chen Y.J., Wei P.C., Lo Y.S., Yao C.F., Kuo M.W, & Chen C.M. (2018). Modeling Alzheimer’s Disease by Induced Pluripotent Stem Cells Carrying APP D678H Mutation. Molecular Neurobiology, 56(6), 3972-3983.
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2

Caspase Activity Assay in Cell Lines

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Both H358 and A549 cells seeded in 6-well plates (5% CO2, 37°C) were pre-incubated overnight before NSC290956 was added to each well. After a period of exposure (24 or 48 h) with various concentrations of NSC290956, cells harvested by trypsinization were washed by PBS and the activity of caspase-3 and caspase-9 was determined by caspase activity assay kit from Abcam Technology Inc. (Beverly, MA), respectively. The absorbance of enzymatically released pNA was measured at 490 nm on a M200 PRO NanoQuantautoreader (TECAN, Switzerland).
Zhang J., Liu Z., Zhao W., Yin X., Zheng X., Liu C., Wang J, & Wang E. (2022). Discovery of Small Molecule NSC290956 as a Therapeutic Agent for KRas Mutant Non-Small-Cell Lung Cancer. Frontiers in Pharmacology, 12, 797821.
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3

Caspase Activity Quantification

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Caspase activity was determined according to caspase activity assay kit manufacturer (Abcam; Cambridge, MA, USA). Briefly, the cell lysates were collected and treated with peptide substrate, and then p-nitroaniline releasing from immunocomplexes was measured at 405 nm.
Su C.M., Chen C.Y., Lu T., Sun Y., Li W., Huang Y.L., Tsai C.H., Chang C.S, & Tang C.H. (2016). A novel benzofuran derivative, ACDB, induces apoptosis of human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress. Oncotarget, 7(50), 83530-83543.
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