Anti staphylococcus aureus antibody biotin

Manufactured by Abcam

Anti-Staphylococcus aureus antibody biotin is a laboratory reagent that binds to and detects the presence of Staphylococcus aureus, a common bacterial pathogen. This antibody is conjugated with biotin, which allows for various detection and labeling applications in research and diagnostic settings.

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Lab products found in correlation

Prolong gold antifade reagent with dapi, by Thermo Fisher Scientific (1 mentions) Streptavidin alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Rabbit anti cleaved caspase 3, by Abcam (1 mentions) Tissue freezing medium, by Leica (1 mentions) Superfrost plus adhesion slides, by Thermo Fisher Scientific (1 mentions) Lsm 700 confocal microscope, by Zeiss (1 mentions) Alexa fluor 647 phalloidin, by Thermo Fisher Scientific (1 mentions) Alexa fluor 568 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Cm1950 cryostat, by Leica (1 mentions) Rabbit anti wide spectrum cytokeratin, by Abcam (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) Poly allylamine hydrochloride pah, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Anti-biotin antibody (10 citations)

2 protocols using anti staphylococcus aureus antibody biotin

Immunohistochemical Staining of Skin Cryosections

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Skin punches were fixed with 4% formaldehyde, overnight at RT. The punches were washed in PBS, embedded in tissue freezing medium (Leica Biosystems), snap-frozen in a dry ice-ethanol bath and stored at –80°C. Leica cryostat CM1950 was used to cut 8 μm-thick cryosections that were mounted onto SuperFrost Plus adhesion slides (Thermo Fisher Scientific). Cryosections were permeabilized with staining buffer (0.3% Triton-100, 3% BSA in PBS) for 1h at RT, in a humidity chamber, and stained with antibodies. The following antibodies were used: rabbit anti-wide spectrum cytokeratin (Abcam), rabbit anti-cleaved caspase-3 (Abcam), anti-Staphylococcus aureus antibody biotin (Abcam), Alexa Fluor 647 Phalloidin (Life technologies), goat anti-rabbit Alexa Fluor 568 (Life Technologies) and streptavidin Alexa Fluor 488 (Life technologies). Primary antibodies were incubated for 1h at RT and, after three washes with PBS, secondary antibodies were added for 30 min at RT, in the dark. After antibody staining, slides were mounted with ProLong Gold Antifade Reagent with DAPI (Life Technologies). Confocal images were acquired using Zeiss LSM 700 confocal microscope.

Cruz A.R., van Strijp J.A., Bagnoli F, & Manetti A.G. (2021). Virulence Gene Expression of Staphylococcus aureus in Human Skin. Frontiers in Microbiology, 12, 692023.

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Staphylococcus aureus Detection on Contact Lenses

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Poly(sodium 4-styrenesulfonate) (PSS; M_w ~200000 g mol⁻¹) (product no. 561967), bovine serum albumin (BSA) (product no. B4287), phosphate buffered saline (PBS pH 7.4) (product no. P3813), and TweenR20 (product no. P9416) were purchased from Sigma–Aldrich. Poly(allylamine hydrochloride) (PAH; M_w ~120000–200000 g mol⁻¹) (product no. 43092) was purchased from Alfa Aesar. Anti-Staphylococcus aureus antibody (product no. ab73962) and Anti-Staphylococcus aureus antibody biotin (product no. ab35192) were purchased from Abcam. Contact lenses (Acuvue Moist 1-day) were purchased from Contact Lenses Canada. Staphylococcus aureus bacteria (product no. 27660) were purchased from American Type Culture Collection (ATCC). Sodium chloride (NaCl) (product no. SX0420) was purchased from Millipore Sigma. Streptavidin coated 5-µm polystyrene beads (product no. SVP-50-5) were purchased from Spherotech Inc. Acrylic glass (PMMA) (product no. 8560K354) was purchased from McMaster-Carr. The LED (product no. C503B-GAN-CB0F0791-ND) was purchased from Digikey and the multimode fiber (product no. FG105LCA) was purchased from Thorlabs. The coverslips were purchased from Fisher Scientific. Reagent grade water was used throughout the experiments.

Veli M, & Ozcan A. (2018). Computational Sensing of Staphylococcus aureus on Contact Lenses Using 3D Imaging of Curved Surfaces and Machine Learning. ACS nano, 12(3), 2554-2559.

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