Rabbit polyclonal anti p akt

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Rabbit polyclonal anti-P-AKT is a laboratory reagent that detects the phosphorylated form of the protein kinase AKT. It is produced by immunizing rabbits with a synthetic phosphopeptide corresponding to a region of AKT that contains a phosphorylated serine or threonine residue.

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P-Akt (239 citations) Anti-Akt (175 citations) Anti-p-Akt (96 citations) Rabbit anti-Akt (13 citations) Polyclonal rabbit (4 citations) Rabbit anti-TM (2 citations)

2 protocols using rabbit polyclonal anti p akt

Protein Expression Analysis of Apoptotic Pathways

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Protein levels of dCK, dGK, caspase-3, caspase-9, Bcl2, Bcl-xL, Bax, Bad, Bid, Bim, AKT, and p-AKT were determined by using standard western blotting techniques [13 (link)]. Mouse monoclonal anti-dCK was developed in the Department of Pediatrics of Mie University School of Medicine [13 (link)]. Rabbit polyclonal anti-dGK antibody (Abgent, San Diego, CA, USA), rabbit polyclonal anti-caspase-3 (Cell Signaling Technology, Beverly, MA, USA), rabbit polyclonal anti-caspase-9 (Cell Signaling Technology), rabbit polyclonal anti-Bcl-2 (Cell Signaling Technology), rabbit polyclonal anti-Bcl-xL (Cell Signaling Technology), rabbit polyclonal anti-Bax (Cell Signaling Technology), rabbit polyclonal anti-Bad (Cell Signaling Technology), rabbit polyclonal anti-Bid (Cell Signaling Technology), rabbit polyclonal anti-Bim (Cell Signaling Technology), rabbit polyclonal anti-AKT (Cell Signaling Technology), rabbit polyclonal anti-P-AKT (Santa Cruz Biotechnology, Inc. Dallas, TX, USA), and anti-actin antibodies (Sigma-Aldrich) were used as primary antibodies [13 (link)].

Yamauchi T., Uzui K., Nishi R., Shigemi H, & Ueda T. (2014). Reduced drug incorporation into DNA and antiapoptosis as the crucial mechanisms of resistance in a novel nelarabine-resistant cell line. BMC Cancer, 14, 547.

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Protein Extract Isolation and Analysis

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Protein extracts were isolated using TRIzol Reagent (Invitrogen) following manufacturer's indications, clarified (12,000xg, 15min, 4°C), denatured and subjected to SDS-PAGE and Western-blot. Antibodies: rabbit polyclonal anti-SET (Abcam), rabbit monoclonal anti-PP2AY307 (Epitomics), rabbit polyclonal anti-AKT, rabbit polyclonal anti-ERK1/2 (Cell Signaling Technology), rabbit polyclonal anti-pAKT, rabbit poly-clonal anti-pERK1/2 (Santa Cruz Biotechnology), rabbit polyclonal anti-CIP2A, and mouse monoclonal anti-β-Actin (Sigma). Proteins were detected with the appropriate secondary antibodies conjugated to alkaline phospatase (Sigma) by chemiluminescence using Tropix CSPD and Tropix Nitro Block II (Applied Biosystems).

Rincón R., Cristóbal I., Zazo S., Arpí O., Menéndez S., Manso R., Lluch A., Eroles P., Rovira A., Albanell J., García-Foncillas J., Madoz-Gúrpide J, & Rojo F. (2015). PP2A inhibition determines poor outcome and doxorubicin resistance in early breast cancer and its activation shows promising therapeutic effects. Oncotarget, 6(6), 4299-4314.

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