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Cd4 bb700 clone sk3

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The CD4-BB700 (clone SK3) is a fluorochrome-conjugated monoclonal antibody that binds to the CD4 receptor on T cells. It is designed for use in flow cytometry applications to identify and quantify CD4-positive cells in biological samples.

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Lab products found in correlation

Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (2 mentions) Pd l1 apc clone mih1, by Thermo Fisher Scientific (2 mentions) Tim 3 bv605, by BioLegend (2 mentions) Ngfr fitc clone me20, by BioLegend (2 mentions) Q8 clone qbend 10, by Thermo Fisher Scientific (2 mentions) Cd4 percp clone sk3, by BioLegend (2 mentions) Lag 3 bv711, by BioLegend (2 mentions) Kaluza software, by Beckman Coulter (2 mentions) Tim 3 pe cy7, by BioLegend (1 mentions) Cd3 apc, by BioLegend (1 mentions) Cd8 bv510 clone sk1, by BioLegend (1 mentions) Cd39 clone a1, by BioLegend (1 mentions) Alexa fluor 647 conjugated antibody, by R&D Systems (1 mentions) Cd27 pe cy7 clone m t271, by BioLegend (1 mentions) Cd8 bv510, by BioLegend (1 mentions) Pd 1 bv605, by BioLegend (1 mentions) Cd3 apc clone sk7, by BioLegend (1 mentions) Pd 1 bv421, by BioLegend (1 mentions)

Close spelling variants of this product

CD4 (clone SK3, CD4-BB700 CD4 (SK3; Clone SK3 BB700

2 protocols using cd4 bb700 clone sk3

1

Multiparametric Flow Cytometry of CAR-T Cells

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Human HER2 and PD-L1 expression on tumor cells was detected using human HER2-PE-CY7 (clone 24D2, Biolegend) and PD-L1-APC (clone MIH1, eBioscience). HER2 4D5 CAR was detected using the anti-trastuzumab idiotype Alexa Fluor 647-conjugated antibody (clone 2661E, R&D system). HER2 4D5 CAR was also detected using human recombinant HER2 protein conjugated with Alex Fluor 647. Human T cells surface phenotype and transduction efficiency were assessed using the following antibodies: NGFR-FITC (clone ME20.4, Biolegend), Q8 (clone QBEND/10, ThermoFisher), CD45-AF700 or CD45-BV605 (clone HI30, Biolegend), CD3-APC (clone SK7, Biolegend), CD4-PerCP (clone SK3, Biolegend), CD4-BB700 (clone SK3, BD Bioscience), CD8-BV510 (clone SK1, Biolegend), CD27-PE-CY7 (clone M-T271, Biolegend), CD28-BV605 (clone CD28.2, Biolegend. Expression of T cell inhibitory receptors was analyzed using PD-1-BV421 or PD-1-BV605 (clone EH12.2H7, Biolegend), TIM-3-BV605 or TIM-3-PE-CY-7 (clone F38-2E2, Biolegend), CD39 (clone A1, Biolegend), LAG-3-BV711 (clone 11C3C65, Biolegend). Live/dead discrimination was determined using LIVE/DEAD fixable Near-IR dead cell stain kit (ThermoFisher). Flow cytometry results were analyzed using Kaluza software (Beckman Coulter).
Yang Z., Li L., Turkoz A., Chen P., Harari-Steinfeld R., Bobbin M., Stefanson O., Choi H., Pietrobon V., Alphson B., Goswami A., Balan V., Kearney A., Patel D., Yang J., Inel D., Vinod V., Cesano A., Wang B., Roh K.H., Qi L.S, & Marincola F.M. (2021). Contextual reprogramming of CAR-T cells for treatment of HER2+ cancers. Journal of Translational Medicine, 19, 459.
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2

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human HER2 and PD-L1 expression on tumor cells was detected using human HER2-PE-CY7 (clone 24D2, Biolegend) and PD-L1-APC (clone MIH1, eBioscience). Human T cells surface phenotype and transduction efficiency were assessed using the following antibodies: NGFR-FITC (clone ME20.4, Biolegend), Q8 (clone QBEND/10, ThermoFisher), CD45-AF700 or CD45-BV605 (clone HI30, Biolegend), CD3-APC (clone SK7, Biolegend), CD4-PerCP (clone SK3, Biolegend), CD4-BB700 (clone SK3, BD Bioscience), CD8-BV510 (clone SK1, Biolegend), CD27-PE-CY7 or BV786 (clone M-T271, Biolegend), CD28-BV605 or BV711 (clone CD28.2, Biolegend). Expression of T cell inhibitory receptors was analyzed using PD-1-BV421 or PD-1-BV605 (clone EH12.2H7, Biolegend), TIM-3-BV605 or TIM-3-PE-CY-7 (clone F38-2E2, Biolegend), CD39 (clone A1, Biolegend), LAG-3-BV711 (clone 11C3C65, Biolegend). Live/dead discrimination was determined using LIVE/DEAD fixable Near-IR dead cell stain kit (ThermoFisher). Mouse cells were assessed using the following antibodies: Flow cytometry results were analyzed using Kaluza software (Beckman Coulter).
Yang Z., Pietrobon V., Bobbin M., Stefanson O., Yang J., Goswami A., Alphson B., Choi H., Magallanes K., Cai Q., Barrett D., Wang B., Qi L.S, & Marincola F.M. (2023). Nanoscale, antigen encounter-dependent, IL-12 delivery by CAR T cells plus PD-L1 blockade for cancer treatment. Journal of Translational Medicine, 21, 158.
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