Blood samples were collected for analysis of inflammatory markers, such as Erythrocyte Sedimentation Rate (ESR) and serum C-Reactive Protein (s-CRP). ACPA subclasses of IgA and IgG were analysed as anti-cyclic citrullinated peptide version 2 (anti-CCP2) and RF subclasses of IgA and IgM RF were analysed with fluorescence immunoassay (Elia, Thermo Fischer Scientific, Uppsala, Sweden) on a Phadia250 instrument (Thermo Fisher Scientific) according to the manufacturer’s instructions. All patients and all autoantibodies were investigated in parallel on one occasion. Cut-off levels for anti-CCP2 IgG and IgA were 7 arbitrary units. For RF IgM it was 9 arbitrary units and for IgA 5 arbitrary units. Serum nitrate/nitrite (NOx) was analysed using a Cayman nitrate/nitrite colorimetric assay kit (Ann Arbor, Michigan 48108 USA). The total coefficient of variation for the NOx assay was 3.4% and the limit of detection was 1 µM/L.
Phadia250 instrument
Manufactured by Thermo Fisher Scientific
Sourced in Sweden
The Phadia250 instrument is a diagnostic system used for the detection and quantification of specific IgE antibodies in patient samples. It utilizes ImmunoCAP technology to perform automated allergy and autoimmune testing. The Phadia250 instrument provides reliable and consistent results, enabling healthcare professionals to make informed decisions about patient treatment and management.
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Lab products found in correlation
2 protocols using phadia250 instrument
1
Inflammatory Markers and Autoantibodies Analysis
2
Quantification of Fecal Calprotectin
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Faecal calprotectin (fCP) was determined using EliA™ Calprotectin 2 (Thermo Fisher Scientific, Uppsala, Sweden), using a Phadia™ 250 instrument according to the manufacturer’s instructions. Cut-off for positive results was 50 mg/kg according to the manufacturer’s instructions. Values below the lower limit of the measuring range (3.8 mg/kg) were assigned an arbitrary value of 1.9 mg/kg.
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