Three different human-derived HL cell lines were used for this study: HDLM-2, L-428 and L-1236 (DSMZ, Braunschweig, Germany). The HDLM-2 and L-428 cell lines were grown in RPMI-1640 medium, supplemented with 20% fetal bovine serum (FBS), 1% L-glutamine, 1% sodium pyruvate, and 1% penicillin–streptomycin (reagents from Invitrogen/Gibco, Burlington, ON, Canada). The L-1236 cell line was grown in RPMI-1640 medium, supplemented with 10% FBS, 1% L-glutamine, 1% sodium pyruvate, and 1% penicillin–streptomycin. Cells were incubated at 37 °C in a humidified atmosphere containing 5% CO2. A concentration of 5 × 106 cells/tissue culture well in 6-well plates (NuncTM Cell Culture Treated Multidishes, ThermoFisher Scientific, Waltham, MA, USA) were used for all cell lines during the inhibition of telomere maintenance pathways assay. This cell number was chosen to simulate the overall number of lymphocytes residing in a regular lymph node [39 (link)].
L 428
Manufactured by Thermo Fisher Scientific
Sourced in Canada, United States
The L-428 is a laboratory equipment product offered by Thermo Fisher Scientific. It is designed to perform a core function, but a detailed and unbiased description cannot be provided while maintaining conciseness. Further information may be available from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Thermo Fisher Scientific (3 mentions)
Nunc cell culture treated multidishes, by Thermo Fisher Scientific (1 mentions)
Hdlm2, by Thermo Fisher Scientific (1 mentions)
Hdlm 2, by Leibniz Institute DSMZ (1 mentions)
L1236, by Leibniz Institute DSMZ (1 mentions)
Kmh 2, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
L 428, by Leibniz Institute DSMZ (1 mentions)
Km h2, by Leibniz Institute DSMZ (1 mentions)
2 protocols using l 428
1
Inhibition of Telomere Maintenance in HL Cells
2
Cultivation of HRS-Derived Cell Lines
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The human HRS-derived cell lines KMH2 and L428 were obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ), Department of Human and Animal Cell Cultures, Braunschweig, Germany in April 2017. Hs445 was obtained from the American Tissue and Cell Collection -ATCC-(Manassas; VA, USA) in March 2017. L428 and KMH2 were cultured in RPMI 1640 medium supplemented with 10 % heat-inactivated fetal bovine serum (FBS) [Gibco, Gaithersburg; MD, USA cat. #: 10437028, lot #: 1623737], 1% L- glutamine (Thermo Fisher Scientific, Waltham; MA, USA, cat#: 25030-081) and penicillin/streptomycin (Thermo Fisher Scientific, cat #: 15140148). Culture media Hs445 were similar but contained 20 % heatinactivated FBS. Cells used in these experiments were from early passage (L428 and KMH2 passage 3, and Hs445, passage 4) and they were treated for mycoplasma and subsequently tested negative as described by Uphoff et al. (1992 Uphoff et al. ( , 2002)) . All cells were maintained in a humid environment of 5% CO 2 at 37 • C.
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