Theaflavin 3 3 digallate

Manufactured by Merck Group

Sourced in Sao Tome and Principe, Macao, India

Theaflavin-3,3'-digallate is a chemical compound found in black tea. It is a type of polyphenol with antioxidant properties. The core function of this compound is to serve as a research tool for studying the biological activities and potential applications of black tea polyphenols.

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Theaflavin (7 citations)

3 protocols using theaflavin 3 3 digallate

Screening of Antiviral Compounds

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Curcumin, tea extract standardized to 85% theaflavins, theaflavin-3,3’-digallate, gallic acid, tannic acid, Andrographis paniculata extract, andrographolide, licorice extract, glycyrrhizic acid, broccoli extract, L-sulforaphane, usnic acid, malic acid, D-limonene, and ammonia chloride with purity between 95–99%, according to the manufacturer, were purchased from Sigma (St. Louis, MO). All other polyphenols and camostat mesylate, with purity between 95–99% according to the manufacturer, were obtained from Cayman Chemical Company (Ann Arbor, MI). For screening study, test compounds were prepared as 10 mg/ml (25% DMSO) working stock solution and for the rest of experiments as 1.0 mg/ml (1% DMSO) and 10 mg/ml (10% DMSO). All antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA). TMPRSS2 recombinant protein was from Creative BioMart (Shirley, NY).

Goc A., Sumera W., Rath M, & Niedzwiecki A. (2021). Phenolic compounds disrupt spike-mediated receptor-binding and entry of SARS-CoV-2 pseudo-virions. PLoS ONE, 16(6), e0253489.

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HPLC Analysis of Polyphenol Composition in Brewed Tea

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The GT and BT polyphenol composition of brewed tea (one bag in 240 mL of boiling water for 5 min) was determined by HPLC with CoulArray electrochemical detection (ESA, Chelmsford, MA) as previously described (GT: mg/L: 855.0±11.8 total phenolics, 396.8 ± 28.3 EGCG, 202.3 ± 10.9 EGC, 52.1 ± 4.7 EC, 62.1 ± 4.6 ECG, 11.8±2.3 gallic acid and 0 THE and BT: mg/L: 661.5±5.8 total phenolics, 28.1±0.8 EGCG, 5.6±0.5 EGC, 8.4±0.2 EC, 15.5±0.3 ECG, 242.1±3.6 gallic acid and 24.6±2.5 THE) [17 ]. All HPLC grade solvents were purchased from Fisher Scientific (Pittsburgh, PA). EGCG, EC, ECG, EGC and black tea extract (mixture of theaflavin, theaflavin-3-monogallate, theaflavin-3’-monogallate and theaflavin-3,3’-digallate) were purchased from Sigma-Aldrich (St.Louis, MO), 4'-O-methyl EGC (4'-MeEGC) and 4"-O-methyl EGCG (4"-MeEGCG) were purchased from Nacalai USA Inc. (San Diego, CA) and theaflavin-3,3’-digallate and theaflavin-3-gallate from Wako Chemicals USA (Richmond VI).

Henning S.M., Wang P., Said J.W., Huang M., Grogan T., Elashoff D., Carpenter C.L., Heber D, & Aronson W.J. (2014). Randomized Clinical Trial of Brewed Green and Black Tea in Men with Prostate Cancer Prior to Prostatectomy. The Prostate, 75(5), 550-559.

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Phytochemical Standards Characterization

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Gallic acid monohydrate (≥98.0%), (−)-epigallocatechin-3-gallate (≥95%),(−)-epigallocatechin (≥95%, HPLC), (−)-epicatechin-3-gallate (≥95%, HPLC), (+)-catechin (≥95%, HPLC), (−)-epicatechin (≥90%, HPLC), caffeine (anhydrous, 99%), theaflavin (≥90%, HPLC), theaflavin-3-gallate (≥95%, HPLC), theaflavin-3′-gallate (≥90%, HPLC), theaflavin-3,3′-digallate (≥90%, HPLC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), potassium persulfate, 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ) and iron(iii) chloride hexa-hydrate were purchased from Sigma-Aldrich, India. Folin-Ciocalteu reagent (LR) was obtained from Himedia (HiMedia Laboratories, India). Acetonitrile (HPLC grade), acetic acid (HPLC grade), sodium carbonate and all other chemicals were procured from Merck KGaA, Darmstadt, Germany.

Deka H., Sarmah P.P., Devi A., Tamuly P, & Karak T. (2021). Changes in major catechins, caffeine, and antioxidant activity during CTC processing of black tea from North East India. RSC Advances, 11(19), 11457-11467.

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