The MTT assay (ab211091; Abcam) was used to assess cell viability. The 1×104 cells were seeded in 96 well plates and cultured under different conditions. After 72 hours, 50 µL of MTT reagent (1 mg/mL) was added to each well and incubated for 1 hour. The density was then quantified with a multimode reader (SYNERGR LX; BioTEK, USA).
Synergr lx
Manufactured by Agilent Technologies
Sourced in United States, China
The SYNERGR LX is a high-performance liquid chromatography (HPLC) system designed for a wide range of analytical applications. It features advanced hardware and software components to deliver reliable and consistent results. The core function of the SYNERGR LX is to separate, identify, and quantify components within a sample mixture.
Automatically generated - may contain errors
2 protocols using synergr lx
1
Evaluating Cell Viability with MTT Assay
2
Cytokine Profiling in Serum
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The levels of interleukin (IL)-6 (E-EL-R0015c; Elabscience, China), IL-1β (E-EL-R0012c; Elabscience), and IL-18 (E-EL-R0567c; Elabscience) in the serum were determined using ELISA kits and the 96-well plates placed into a multimode reader (SYNERGR LX; BioTEK).
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