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5 protocols using decanoyl chloride

1

Decyl Maltoside Protein Purification

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Decyl β-D-maltoside (DM) (Sigma 850521P, 99% pure); n-dodecyl-β-D-maltoside (DDM) (Sigma, D4641, 98% pure); L-histidine (Sigma H8000, 99% pure); imidazole (Sigma I202, 99% pure); NiCl2 (Sigma, 339,350 98% pure); ZnCl2 (Sigma, 208,086, 98% pure); CuCl2 (Sigma, 751,944, 99% pure); FeCl2 (Sigma, 372,870, 98% pure); EDTA (Sigma, 03,609, 99% pure); PEG-6000 (Sigma, 89,510), Tris (Sigma, 93,352, 99% pure); tetrahydrofuran (THF) (Sigma, 186,562, 99.9% pure); decanoyl chloride (Sigma, 140,295, 98% pure); KOH (Sigma, 757,551, 99% pure).
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2

Synthesis of Functionalized Silica Nanoparticles

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L-tryptophan (98%), decanoyl chloride (98%), sodium hydroxide (97%), sodium bicarbonate (97%), 3-aminopropyl trimethoxysilane (97%), tetraethyl orthosilicate (98%), butyric acid (99%), cetyltrimethylammonium bromide (99%), and dichloromethane (99.8%) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Tetrahydrofuran (TFH, 99.5%), hydrochloric acid (35% w/w), oleic acid (99%), palmitic acid (99%), citric acid (99%), ammonia solution (32% w/w), iron (III) chloride (97%), iron (II) chloride tetrahydrate (99%), and sodium chloride (99.5%) were supplied by Scharlab (Barcelona, Spain).
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3

Synthesis of Functionalized Silica Nanoparticles

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Cilastatin (95%) was purchased from Ambeed, Inc. (Arlington Hts, IL, USA) Oleoyl chloride (≥89%), decanoyl chloride (98%), tetrahydrofuran (THF, ≥99.0%), (3-aminopropyl)triethoxysilane (APTES, 98%), (3-aminopropyl)trimethoxysilane (APTMS, 97%), tetraethyl orthosilicate (TEOS, 98%), and hydrochloric acid (35% w/w) were obtained from Sigma-Aldrich (Madrid, Spain).
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4

Synthesis and Purification of Fatty Acid Derivatives

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Decanoylchloride (N10), laurylchloride (N12), myristoyl-chloride (N14), palmitoylchloride (N16), stearoylchloride (N18), tromethamine hydrochloride (TM), 8-anilinonapthalene sulfonic acid (ANS), laurdan and prodan were purchased from Sigma, India. All other reagents were of reagent grade and were obtained from local suppliers. Solvents were distilled and dried prior to usage.
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5

Synthetic Organic Procedures and Analytical Methods

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All solvents were distilled prior
to use, and all reagents were
used without further purification unless otherwise noted. All synthetic
reactions were conducted under a nitrogen or argon atmosphere. Silica
gel 60 Å, 40–75 μm (200–400 mesh), was used
for column chromatography. Aluminum-backed silica gel 200 μm
plates were used for thin-layer chromatography (TLC). 1H NMR spectra were obtained using either a 300 MHz spectrometer or
a 500 MHz spectrometer with tetramethylsilane (TMS) as the internal
standard, and 13C NMR spectra were obtained using a 500
MHz spectrometer. High-resolution mass spectrometry (HRMS) spectra
were measured on a time-of-flight (TOF) instrument by electrospray
ionization (ESI). Decanamide, decanoyl chloride, γ-aminobutyric
acid, and bis-(trimethylsilyloxy)cyclobutene were purchased from Sigma-Aldrich
(St. Louis, MO). All other reagents for biochemical assays were purchased
at the highest quality available.
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