Anti traf6 antibody

Manufactured by Abcam

Sourced in United States

Anti-TRAF6 antibody is a primary antibody that recognizes the TRAF6 (Tumor Necrosis Factor Receptor-Associated Factor 6) protein. TRAF6 is an adaptor protein that plays a crucial role in various signaling pathways, including those involved in immune response, inflammation, and cell survival. This antibody can be used for the detection and study of TRAF6 in various experimental applications.

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Lab products found in correlation

Horseradish peroxidase conjugated secondary antibody, by Santa Cruz Biotechnology (1 mentions) Tgf β, by Santa Cruz Biotechnology (1 mentions) Anti β actin, by Merck Group (1 mentions) Immobilon p membrane, by Merck Group (1 mentions) Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions) Anti k63 ubiquitin, by Merck Group (1 mentions) Phospho jnk, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Anti traf6, by Santa Cruz Biotechnology (1 mentions) P44 42 mapk, by Cell Signaling Technology (1 mentions) Phospho iκbα, by Cell Signaling Technology (1 mentions) Anti β actin antibody, by Abcam (1 mentions) Anti csrc antibody, by Abcam (1 mentions) Anti p65 antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

TRAF6 (40 citations) Anti-TRAF6 (17 citations) TRAF6 antibody (2 citations)

4 protocols using anti traf6 antibody

Investigating FGFR1 and TRAF6 Signaling Pathways

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The general procedure for Western blot analysis was described in our previous publication.17 (link) Antibodies against P-FGFR1, FGFR1, transforming growth factor-β (TGF-β), collagen (col) IV, Bax, Bcl-2, IκB-α, P-TGF-β activated kinase 1 (TAK1), TAK1, GAPDH and the secondary horseradish peroxidase-conjugated antibody were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Antibody against Ub was obtained from Cell Signaling Technology (CST, CA, USA). Antibody against TNF receptor associated factor-6 (TRAF6) was obtained from Abcam (Cambridge, MA, USA).
For immunoprecipitation experiments, cell extracts were incubated with anti-TRAF6-antibody for 4 h and were then precipitated with protein G-Sepharose beads at 4 °C overnight. FGFR1 and TRAF6 levels were detected by Western blotting using specific antibodies.

Chen X., Zhang X., Xu J., Zhao Y., Bao J., Zheng Z, & Han J. (2020). AZD4547 Attenuates Lipopolysaccharide-Induced Acute Kidney Injury by Inhibiting Inflammation: The Role of FGFR1 in Renal Tubular Epithelial Cells. Drug Design, Development and Therapy, 14, 833-844.

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Investigating TRAF6 Ubiquitination in DT40 Cells

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WT DT40 cells and TAX1BP1^−/−/− cells were incubated for indicated time after CD40 activation and lysed in RIPA buffer. Lysates were incubated with anti-TRAF6 antibody (abcam) or relative non-immunized IgG for 3 h at 4 °C followed by protein G Sepharose for another 2 h. Proteins were detected by anti-TRAF6 (Santa Cruz Biotechnology), anti-β-actin (SIGMA) and anti-K63 Ubiquitin (Millipore).
Whole-cell lysates from DT40 cells were separated by SDS-PAGE gels and transferred to Immobilon-P membrane (Millipore). The membranes were blotted with the following antibodies: I-κBα, Phospho-I-κBα, JNK, Phospho-JNK, p44/42 MAPK, phospho-p44/42 MAPK (Cell Signaling), TAX1BP1 (Abnova), β-actin (SIGMA).

Matsushita N., Suzuki M., Ikebe E., Nagashima S., Inatome R., Asano K., Tanaka M., Matsushita M., Kondo E., Iha H, & Yanagi S. (2016). Regulation of B cell differentiation by the ubiquitin-binding protein TAX1BP1. Scientific Reports, 6, 31266.

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Immunoprecipitation of TRAF6 and c-Src

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RAW264.7 cells were treated with NBIF and then underwent homogenization and centrifugation; the supernatant was collected and incubated with beads bounded to the specific (TRAF6 or c‐Src) antibody. The mixture was then centrifuged with the supernatant discarded. The beads were washed with washing buffer to collect the protein complex. Subsequently, sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and Western blot analysis were followed. The antibodies were used as follows: anti‐TRAF6 antibody (Abcam), anti‐c‐Src antibody (Abcam) and anti‐β‐actin antibody (Abcam).

Chen H., Fang C., Zhi X., Song S., Gu Y., Chen X., Cui J., Hu Y., Weng W., Zhou Q., Wang Y., Wang Y., Jiang H., Li X., Cao L., Chen X, & Su J. (2020). Neobavaisoflavone inhibits osteoclastogenesis through blocking RANKL signalling‐mediated TRAF6 and c‐Src recruitment and NF‐κB, MAPK and Akt pathways. Journal of Cellular and Molecular Medicine, 24(16), 9067-9084.

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Western Blot and qRT-PCR Techniques

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Anti-p65 antibody was purchased from Cell signaling (Cat. #8242, USA), and anti-TRAF6 antibody was obtained from Abcam (Cat. 33915, UK). PrimerScript 1^st strand cDNA synthesis Kit (Cat. 6210) and SYBR Green Master Mix kit (Cat. RR420) were purchased from Takara (Japan).

He X., Pu G., Tang R., Zhang D, & Pan W. (2014). Activation of Nuclear Factor Kappa B in the Hepatic Stellate Cells of Mice with Schistosomiasis Japonica. PLoS ONE, 9(8), e104323.

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