0.4 µM polyester transwell inserts (VWR) were coated with 100 µg/ml Collagen IV (Sigma-Aldrich C5533) for 4 hours. ARPE-19 cells were seeded at a density of 1.7x105 cells per cm2 in DMEM F-12 Ham containing 10% (FBS). Two days later medium was replaced with complete medium containing 1% FBS and replenished twice weekly for 4–6 weeks.
Polyester transwell inserts
Manufactured by Avantor
Polyester transwell inserts are a type of laboratory equipment used in cell culture studies. They are permeable membrane-based supports that allow for the separation and co-culture of different cell types within a single system.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using polyester transwell inserts
1
ARPE-19 Cell Culture on Collagen IV
2
ARPE-19 Cell Culture on Collagen IV
Check if the same lab product or an alternative is used in the 5 most similar protocols
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0.4 µM polyester transwell inserts (VWR) were coated with 100 µg/ml Collagen IV (Sigma-Aldrich C5533) for 4 hours. ARPE-19 cells were seeded at a density of 1.7x105 cells per cm2 in DMEM F-12 Ham containing 10% (FBS). Two days later medium was replaced with complete medium containing 1% FBS and replenished twice weekly for 4–6 weeks.
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