Mouse interferon beta

Human interferon beta, gamma, and TNF-α were purchased from PeproTech Inc. (NJ, USA). Murine interferon-gamma was obtained from PeproTech Inc. (NJ, USA), and mouse interferon beta was purchased from PBL Assay Science (12405-1). Cytokines reconstitution was handled according to the manufacturer’s recommendations. Briefly, the lyophilized powder was reconstituted in water to a concentration of 1mg/mL, then further in 0.2% BSA to a final concentration of carrier 0.1% BSA and cytokines to 0.5mg/mL or up to 2.5x10⁶ units/mL. Human and murine recombinant cytokines were routinely applied at concentrations: hrIFN-β (10 ng/mL), hrIFN-γ (100ng/mL), hrTNF-α (10ng/mL), mrIfn-β (1000U/mL), mrIfn-γ (50 or 100U/mL). For gene expression analysis, cytokines were added into a standard growth media and incubated for 12 hours prior to the collection of an experiment.

Human interferon beta, gamma, and TNF-α were purchased from PeproTech Inc. (NJ, USA). Murine interferon-gamma was obtained from PeproTech Inc. (NJ, USA), and mouse interferon beta was purchased from PBL Assay Science (12405-1). Cytokines reconstitution was handled according to the manufacturer’s recommendations. Briefly, the lyophilized powder was reconstituted in water to a concentration of 1mg/mL, then further in 0.2% BSA to a final concentration of carrier 0.1% BSA and cytokines to 0.5mg/mL or up to 2.5x10⁶ units/mL. Human and murine recombinant cytokines were routinely applied at concentrations: hrIFN-β (10 ng/mL), hrIFN-γ (100ng/mL), hrTNF-α (10ng/mL), mrIfn-β (1000U/mL), mrIfn-γ (50 or 100U/mL). For gene expression analysis, cytokines were added into a standard growth media and incubated for 12 hours prior to the collection of an experiment.