Dle enzyme

Manufactured by Bionano Genomics

The DLE enzyme is a laboratory tool used in genomic analysis. It serves as a restriction endonuclease, cleaving DNA molecules at specific recognition sequences. The core function of the DLE enzyme is to facilitate the fragmentation of DNA samples, enabling further processing and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Blood and cell culture dna isolation kit, by Bionano Genomics (1 mentions)

Spelling variants of this product

DLE-1 enzyme (7 citations)

2 protocols using dle enzyme

Lipo863B Genome Optical Mapping

Check if the same lab product or an alternative is used in the 5 most similar protocols

The genomic DNA from Lipo863B was used for BioNano optical mapping. gDNA was extracted using BioNano Blood and Cell Culture DNA Isolation Kit (80004). Next, the homogenized gDNA was directly labeled using DLE enzyme (BioNano, 80005) according to the BioNano Prep Direct Label and Stain (DLS)-30206 protocol. The labeled and stained genomic DNA was then loaded onto a Saphyr chip for optical data collection.

Liu T., Wang J., Yang H., Jin Q., Wang X., Fu Y., Luan Y., Wang Q., Youngblood M.W., Lu X., Casadei L., Pollock R, & Yue F. (2023). Enhancer Coamplification and Hijacking Promote Oncogene Expression in Liposarcoma. Cancer Research, 83(9), 1517-1530.

+ Open protocol

+ Expand

Optical Mapping of Muscle Genome

Check if the same lab product or an alternative is used in the 5 most similar protocols

Genomic DNA from one Tübingen female muscle was extracted for BioNano optical mapping. DNA extraction was done according to the BioNano Prep Animal Tissue DNA Isolation Fibrous Tissue Protocol-30071. The homogenized genomic DNA was then directly labeled by DLE enzyme (BioNano, 80005) according to the BioNano Prep Direct Label and Stain (DLS)-30206 protocol. The labeled and stained genomic DNA was then loaded onto a Saphyr chip, and 407 Gb data was collected for genome assembly.

Yang H., Luan Y., Liu T., Lee H.J., Fang L., Wang Y., Wang X., Zhang B., Jin Q., Ang K.C., Xing X., Wang J., Xu J., Song F., Sriranga I., Khunsriraksakul C., Salameh T., Li D., Choudhary M.N., Topczewski J., Wang K., Gerhard G.S., Hardison R.C., Wang T., Cheng K.C, & Yue F. (2020). A map of cis-regulatory elements and 3D genome structures in zebrafish. Nature, 588(7837), 337-343.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!