Twist1

Manufactured by GeneTex

Sourced in United States, Japan

TWIST1 is a protein that functions as a transcriptional regulator. It plays a role in the regulation of embryonic development, cell lineage determination, and various cellular processes.

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Lab products found in correlation

Fibronectin, by GeneTex (3 mentions) E cadherin, by GeneTex (3 mentions) Snail, by GeneTex (2 mentions) Vimentin, by GeneTex (2 mentions) N cadherin, by GeneTex (2 mentions) E2f1 antibody, by Cell Signaling Technology (1 mentions) Wnt5a, by GeneTex (1 mentions) Nanog, by GeneTex (1 mentions) Pik3c3, by Cell Signaling Technology (1 mentions) Snail, by Cell Signaling Technology (1 mentions) β actin, by Merck Group (1 mentions) Rn003m, by MBL Life Science (1 mentions) N cadherin, by BD (1 mentions) Fitc conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Rhodamine conjugated anti mouse igg, by Jackson ImmunoResearch (1 mentions) Pd l1, by GeneTex (1 mentions) Cd133, by GeneTex (1 mentions) Snail1, by GeneTex (1 mentions)

Spelling variants of this product

Twist1/2 (3 citations) Anti-Twist1/2 (3 citations)

4 protocols using twist1

Comprehensive Protein Analysis Techniques

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Western blot analysis and immunohistochemical (fluorescence) staining were conducted as previously described.^{14 (link), 15 (link)} The primary antibodies used in this study were actin monoclonal antibody (1:5000, Merck, Darmstadt, Germany), E2F1 antibody (1:1000, Cell Signaling Technology, Beverly MA, USA), GFP monoclonal antibody (1:200, Upstate, Lake Placid, NY, USA), FITC-conjugated anti-rabbit, rhodamine-conjugated anti-mouse, alkaline phosphatase–conjugated anti-rabbit antibody (1:500, Jackson ImmunoResearch Laboratories, West Grove, PA, USA), SPZ1, TWIST1, AKT, Ki67, HIF-1, Snail, Slug, Wnt5a, ZEB1, BMI1, fibronectin, E-cadherin rabbit polyclonal antibody (1:1000; GeneTex, Irvine, CA, USA). ERK1/2, PI3K monoclonal antibodies (Santa Cruz Biotechnology, Dallas, TX, USA). CD133, CD44, N-cadherin, vimentin rabbit polyclonal antibody (1:500, Abcam, Cambridge, MA, USA) All of the experiments were repeated at least three times.

Wang L.T., Chiou S.S., Chai C.Y., Hsi E., Chiang C.M., Huang S.K., Wang S.N., Yokoyama K.K, & Hsu S.H. (2017). Transcription factor SPZ1 promotes TWIST-mediated epithelial–mesenchymal transition and oncogenesis in human liver cancer. Oncogene, 36(31), 4405-4414.

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Comprehensive Histone and EMT Marker Analysis

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Primary antibodies for H3K4me3 (GTX128954), H3K9me3 (GTX121677), H3K27me3 (GTX121184), H3K36me3 (GTX54109), H3K79me3 (GTX54111), histone H3 (GTX122148), MMP2 (GTX104577), MMP3 (GTX100723), MMP9 (GTX100458), N-cadherin (GTX101141), E-cadherin (GTX100433), vimentin (GTX100619), Snail (GTX100754), TWIST-1 (GTX60776), Nanog (GTX100863), OCT4 (GTX101497), SOX2 (GTX101507), ABCB1 (GTX108354), ABCG2 (GTX100437) and ABCC1 (GTX88673) were purchased from GeneTex International Corporation (Hsinchu City, Taiwan). Anti-mouse and anti-rabbit IgG-conjugated horseradish peroxidase and rabbit polyclonal antibodies speci c for β-actin (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany; cat. no. SI-A5441; 1:10,000) were used in this study. All other chemicals were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany).

Chang S.L., Lee C.W., Yang C.Y., Lin Z.C., Peng K.T., Liu S.C., Wang S.W., Tsai H.C., Fong Y.C., Lai C.Y., Huang Y.L., Tsai C.H., Ko C.Y., Liu J.F, & Tang C.H. (2023). IOX-1 suppresses metastasis of osteosarcoma by upregulating histone H3 lysine trimethylation. Biochemical pharmacology, 210.

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Protein Expression Profiling Protocol

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Protein expression was measured as previously described [29 (link)], using Western blotting with antibodies (Abs) to anti-EIF2C2/AGO2 mouse monoclonal antibody (RN003M, MBL International, Nagoya, Japan), PIK3C3 (#4263; Cell Signaling Technology, Beverly, MA, USA), LC3 (PM036; MBL, Nagoya, Japan), P62 (PM045; MBL), Twist1 (GTX60776; GeneTex, Irvine, CA, USA), Snail (#3879; Cell Signaling Technology), ATG5 (#2630; Cell Signaling Technology), N-cadherin(610,920, BD Transduction Labs, San Diego, CA, USA), E-cadherin (GTX100443; GeneTex), Vimentin (GTX100619; Gentex), Fibronectin (GTX112794; GeneTex) and, β-actin (A5441; Sigma-Aldrich, St. Louis, MO, USA). Full blots were provided in the supplementary information (Supplementary Fig. S6).

Chu C.A., Lee C.T., Lee J.C., Wang Y.W., Huang C.T., Lan S.H., Lin P.C., Lin B.W., Tian Y.F., Liu H.S, & Chow N.H. (2019). MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway. EBioMedicine, 43, 270-281.

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Comprehensive Antibody Staining Protocol

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Western blotting and immunohistochemical (fluorescence) staining were performed as described previously.^[¹⁰, ²⁹^] The primary antibodies used in this study were PIINP and Actin polyclonal antibodies (1:5000 dilution; Sigma‐Aldrich), GFP monoclonal antibodies (1:500 dilution; Upstate, NY), FITC‐conjugated anti‐rabbit IgG, rhodamine‐conjugated antimouse IgG, and alkaline phosphatase‐conjugated anti‐rabbit IgG antibody (1:500 dilution; Jackson ImmunoResearch Laboratories), and CYP1B1 rabbit polyclonal antibody (1:200 dilution; Santa Cruz Biotechnology). The AHR goat polyclonal antibody (1:200 dilution; Santa Cruz Biotechnology). The Core protein, ISX, IDO1, TDO2, CD86, PD‐L1, N‐cadherin, E‐cadherin, Fibronectin, Vimentin, BMI1, CD133, TWIST1, Snail1, Slug, and ZEB1 (1:500 dilution) primary antibody was obtained from GeneTex International Corp. The Onecut1, GADD45G, PDGFA, SOX13, FOCX1, and LPIN1(1:500 dilution) primary antibody was obtained from Elabscience. All of the experiments were repeated at least 3 times.

Wang L., Wang S., Chiou S., Tsai J., Chai C., Tseng L., Lee J., Lin M., Huang S, & Hsu S. (2023). HCV Core Protein–ISX Axis Promotes Chronic Liver Disease Progression via Metabolic Remodeling and Immune Suppression. Advanced Science, 10(23), 2300644.

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