Flow jo version x flow cytometry analysis software

Cells were seeded at a density of ~3x10 5 /well into 6-well plates and subsequently transfected with 200 pmol miR-24-2 mimic, inhibitor, NC or inhibitor NC. After 48 h, cells were harvested and washed twice with cold PBS. Cells were subsequently resuspended in 100 µl 1X binding buffer and 5 µl annexin V-fluorescein isothiocyanate, following which 3 µl propidium iodide (all from Invitrogen; Thermo Fisher Scientific, Inc.) was added into each cell suspension. A total of 15 min later, 400 µl binding buffer was added to each tube. A COULTER ® EPICS ® XL™ Flow Cytometer (Beckman Coulter, Inc., Brea, CA, USA) was subsequently used to analyze the apoptosis rate. FlowJo (version X) flow cytometry analysis software (FlowJo LLC, Ashland, OR) was used in the study. Statistical analysis. A paired t-test was used to compare the expression levels of miR-24-2 in matched tissues. An unpaired Student's t-test was used to analyze assays characterizing the phenotypes of cells. All statistical analyses were performed using SPSS software version 19.0 (IBM SPSS, Armonk, NY, USA) and data are presented as the mean ± standard deviation. P<0.05 was considered to indicate a statistically significant difference.