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Softmax pro spectrometer

Manufactured by Molecular Devices
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Sourced in United States

The SoftMax® Pro spectrometer is a versatile laboratory instrument designed for absorbance, fluorescence, and luminescence measurements. It provides precise and reliable data collection for a wide range of applications in life science research and analysis.

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Lab products found in correlation

Mucin assay kit, by Cosmo Bio (2 mentions) Xfluor4, by Tecan (1 mentions)

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SoftMax Pro (266 citations)

2 protocols using softmax pro spectrometer

1

Quantification of Fecal Mucin and Cecal IgA

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Mucin was extracted from each 100 mg fecal sample. Fecal mucin contents were determined using a fecal Mucin Assay Kit (Mucin Assay Kit, Cosmo Bio, Co., Ltd., Tokyo, Japan). A fluorometric assay discriminated O-linked glycoproteins (mucins) from N-linked glycoproteins. Fluorescence was measured using a SoftMax® Pro spectrometer (Molecular Devices, CA, USA). Total IgA was extracted from 50 mg of cecum samples and quantified using a mouse IgA ELISA quantitation kit (Immundiagnostik, Bensheim, Germany) as specified in the manufacturer’s instructions. The reaction products were determined from absorbance at 450 nm using XFluor4 (Tecan, Zurich Switzerland), and IgA was qualified.
Takahashi M., Takahashi K., Abe S., Yamada K., Suzuki M., Masahisa M., Endo M., Abe K., Inoue R, & Hoshi H. (2020). Improvement of Psoriasis by Alteration of the Gut Environment by Oral Administration of Fucoidan from Cladosiphon Okamuranus. Marine Drugs, 18(3), 154.
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2

Fecal Mucin Quantification Assay

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Mucin was extracted from each 100 mg fecal sample. Fecal mucin contents were determined using a fecal Mucin Assay Kit (Mucin Assay Kit, Cosmo Bio, Co., Ltd., Tokyo, Japan). A fluorometric assay discriminated O-linked glycoproteins (mucins) from N-linked glycoproteins. Fluorescence was measured using a SoftMax®Pro spectrometer (Molecular Devices, CA, USA).
Amorim Neto D.P., Bosque B.P., Pereira de Godoy J.V., Rodrigues P.V., Meneses D.D., Tostes K., Costa Tonoli C.C., Faustino de Carvalho H., González-Billault C, & de Castro Fonseca M. (2022). Akkermansia muciniphila induces mitochondrial calcium overload and α -synuclein aggregation in an enteroendocrine cell line. iScience, 25(3), 103908.
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